Blood Markers of Mobile Source Particle Exposure

移动源粒子暴露的血液标记

• 批准号: 7146757
• 负责人: ERIC GARSHICK
• 金额: $ 21.78万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7146757
• 关键词: air pollution; biomarker; blood chemistry; blood toxicology; carbopolycyclic compound; cardiovascular disorder risk; clinical research; data collection methodology /evaluation; engine exhaust; environmental exposure; environmental toxicology; human population study; human subject; inflammation; medical outreach /case finding; method development; naphthoquinones; occupational hazard; particle; particle counter; sample collection; vascular endothelium

DESCRIPTION (provided by applicant): Large population studies of fine particulate air pollution have shown a link with heart disease, but for practical reasons they have not included direct assessment of biochemical risk factors or personal exposures. In this Exploratory/Developmental Research application we develop the methodology to collect blood samples and recruit study participants from a national cohort of unionized trucking company workers who have regular occupational exposures to fine particulate matter (PM) from motor vehicle exhaust. With the cooperation of the Teamsters we propose to assess the rate that unionized trucking company workers agree to provide a blood sample and complete a health questionnaire for a health study. Phlebotomy and study recruitment sites will be located in Teamster union halls near large trucking terminals in Chicago, IL and Carlisle, PA that employ 3,481 workers. The demographics and exposure distribution of subjects who provide a blood sample will be compared to workers who do not provide a blood sample. This strategy of locating phlebotomy centers in Union halls will be compared to the results from a companion study where another group of Teamsters are recruited by mail and requested to provide a blood sample by overnight mail using a blood collection kit. Preliminary information regarding the relationship between combustion particle exposures (based on job title and terminal location) and blood markers of inflammation that include C-reactive protein (CRP, measured using a high sensitivity assay), fibrinogen, interleukin-6 (IL-6), and markers of vascular endothelial activation and inflammation (soluble intercellular adhesion molecule-1 (slCAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1)) will be obtained. Cross-sectional relationships between combustion particle exposures, blood markers of inflammation, and concentrations of 1,2-naphthoquinone and 1,4- naphthoquinone albumin adducts, specific biomarkers of polycyclic aromatic hydrocarbons (PAHs) will be assessed. This approach will offer the possibility of prospective personal monitoring for a large population, greatly increasing the power to detect health effects and link them to personal risk factors and exposure to motor vehicle exhaust. The results from the proposed exploratory and developmental study will aid us in designing a prospective study to determine the relationship between PM exposures from motor vehicles and cardiovascular disease. This is of public health importance because large segments of the general US population have exposures to fine particles similar to trucking company worker exposures.

描述（由申请人提供）：对细颗粒空气污染的大规模人群研究表明与心脏病有关，但出于实际原因，他们没有包括对生化风险因素或个人暴露的直接评估。在这个探索性/发展性研究应用程序中，我们开发了一种方法来收集血液样本，并从一个全国性的工会卡车运输公司工人队列中招募研究参与者，这些工人定期职业暴露于机动车尾气中的细颗粒物（PM）。在卡车司机协会的合作下，我们建议评估加入工会的卡车运输公司工人同意提供血液样本和完成健康调查问卷的比率。放血术和研究招募地点将位于伊利诺伊州芝加哥和宾夕法尼亚州卡莱尔大型货运码头附近的Teamster工会霍尔斯，雇用3，481名工人。将提供血样的受试者的人口统计学和暴露分布与未提供血样的工作人员进行比较。将在联合霍尔斯内定位放血中心的策略与一项同伴研究的结果进行比较，该研究通过邮件招募了另一组卡车司机，并要求他们使用采血套件通过隔夜邮件提供血样。关于燃烧粒子暴露与（基于职位和终点位置）和炎症的血液标志物，包括C反应蛋白（CRP，使用高灵敏度测定法测量）、纤维蛋白原、白细胞介素-6（IL-6）以及血管内皮活化和炎症的标志物将获得可溶性细胞间粘附分子-1（sICAM-1）和可溶性血管细胞粘附分子-1（sVCAM-1）。将评估燃烧颗粒暴露、炎症的血液标志物和1，2-萘醌和1，4-萘醌白蛋白加合物、多环芳烃（PAH）的特定生物标志物的浓度之间的横截面关系。这一方法将提供对大量人口进行前瞻性个人监测的可能性，大大提高了检测健康影响并将其与个人风险因素和机动车废气接触联系起来的能力。所提出的探索性和发展性研究的结果将有助于我们设计一项前瞻性研究，以确定机动车PM暴露与心血管疾病之间的关系。这对公共卫生具有重要意义，因为美国大部分普通人群都暴露于类似于卡车运输公司工人暴露的细颗粒物。