Oxygen Gas Perfusion System for Pancreas Preservation

用于保存胰腺的氧气灌注系统

• 批准号: 7056605
• 负责人: LINDA A TEMPELMAN
• 金额: $ 24.99万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056605
• 关键词: diabetes mellitus; electrochemistry; laboratory mouse; pancreas; pancreatic islet transplantation; perfusion; portable biomedical equipment; swine; technology /technique development; tissue /organ preservation

DESCRIPTION (provided by applicant): The goal of the proposed work is to develop a substantially improved technique of preserving the human pancreas in order to improve the quality of the isolated human islets that are harvested from it for use in human islet transplantation for Type 1 diabetes. Currently, human pancreata are transported with the two layer method (TLM) of storage (8¿C), which uses an oxygenated perfluorocarbon solution as an external oxygen reservoir. While the TLM is widely reported to improve islet quality, there are still insufficiencies in the number of viable islets acquired. This is partially attributable to viable islet loss during pancreas storage. Preliminary calculations and experiments indicate that external oxygenation of the pancreas only benefits tissue in the outer rim of the organ leaving the majority of the volume of the pancreas without oxygenation (85-95%). The proposed work will use a novel method of oxygen delivery to the interior of the organ. Another innovative component of the research and development is to develop a system to generate and regulate oxygen delivery to the organ using a portable electrochemical oxygen concentrator/generator. The proposed electrochemical oxygen concentrator uses electrical power to concentrate oxygen from air and release it as pure, humidified oxygen that can be blended with ambient air to give the most efficacious pO2 for the proposed preservation technique. Delivery of gaseous oxygen to the interior of the organ is less complex than full liquid perfusion of the organ. Also the proposed system will be designed to be portable since the main "storage" time of the pancreas before islet isolation is during its transportation to a facility that specializes in harvesting islets from pancreata. Oxygen will be continuously generated at the desired flow rate and pressure without the need for transporting pressurized gas cylinders. This proposed method has the potential to effect a breakthrough, rather than an incremental improvement, in the quality of islets harvested from the pancreas. The effective treatment of serious cases of Type I Diabetes through pancreatic islet transplant is a high priority in cutting-edge healthcare research, and the proposed work will contribute to the advancement of the field. COMMERCIAL POTENTIAL The preservation system equipment could be a specialty commercial product, supplied to the major organ harvest and transplantation centers. Alternatively, the transportation and preservation of pancreata, using the proposed technique, could be a commercial service. The techniques and equipment could be applicable to the preservation of other organs. KEYWORDS pancreas, pancreata, Type I Diabetes, organ transport, islet transplantation, electrochemical oxygen concentration.

描述（由申请人提供）：所提出的工作的目标是开发一种实质性改进的保存人胰腺的技术，以提高从其收获的分离的人胰岛的质量，用于1型糖尿病的人胰岛移植。目前，人类胰腺是用双层储存法（TLM）（8 ℃）运输的，该方法使用含氧的全氟化碳溶液作为外部储氧器。虽然TLM被广泛报道可以改善胰岛质量，但在获得的活胰岛数量方面仍然存在不确定性。这部分归因于胰腺储存期间存活胰岛的损失。初步计算和实验表明，胰腺的外部氧合仅有益于器官外缘的组织，留下大部分胰腺体积没有氧合（85-95%）。拟议的工作将使用一种新的氧气输送到器官内部的方法。研究和开发的另一个创新组成部分是开发一种使用便携式电化学氧气浓缩器/发生器产生和调节氧气输送到器官的系统。所提出的电化学氧气浓缩器使用电力从空气中浓缩氧气，并将其释放为纯的、加湿的氧气，该氧气可以与环境空气混合，以提供所提出的保存技术的最有效的pO 2。将气态氧输送到器官内部不如器官的全液体灌注复杂。此外，所提出的系统将被设计为便携式的，因为在胰岛分离之前胰腺的主要“储存”时间是在其运输到专门从胰腺收获胰岛的设施期间。氧气将以所需的流速和压力连续产生，而无需运输加压气瓶。该方法有可能在从胰腺收获的胰岛质量方面取得突破，而不是逐步改善。通过胰岛移植有效治疗I型糖尿病的严重病例是尖端医疗保健研究的重中之重，拟议的工作将有助于该领域的进步。商业潜力保存系统设备可以是一个专业的商业产品，提供给主要的器官收获和移植中心。或者，使用所提出的技术，胰腺的运输和保存可以是商业服务。该技术和设备可适用于其他器官的保存。关键词胰腺，胰腺，I型糖尿病，器官转运，胰岛移植，电化学氧浓度。