Low-Cost Carbon Dioxide Generator for Cylinder-Free Cell Culture Incubators

用于无圆筒细胞培养箱的低成本二氧化碳发生器

• 批准号: 7480172
• 负责人: LINDA A TEMPELMAN
• 金额: $ 14.67万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2009-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7480172
• 关键词: Address; Air; Antibody Formation; Area; Basic Science; Bicarbonates; Biological Sciences; Buffers; Carbon; Carbon Dioxide; Cell physiology; Cells; Cellular Assay; Chemicals; Construction Materials; Culture Media; Cultured Cells; Development; Devices; Disease; Excision; Gases; Generations; Goals; Grant; Housing; Humidity; Incubators; Islets of Langerhans; Laboratories; Liquid substance; Mammalian Cell; Manufacturer Name; Marketing; Mechanics; Medical; Medical Research; Membrane; Methanol; Methods; Minnesota; Nitrogen; Numbers; Operative Surgical Procedures; Organ; Oxygen; Permeability; Phase; Platinum; Play; Pliability; Positioning Attribute; Process; Production; Property; Public Health; Range; Rate; Reaction; Research; Research Personnel; Residual state; Role; Safety; Sales; Skin; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solutions; Standards of Weights and Measures; Stem cells; Stream; Structure; Surface; System; Technology; Testing; Tissue Engineering; Tissues; Transplantation; United States National Institutes of Health; Universities; Vaccine Production; Water; anticancer research; aqueous; base; blastomere structure; catalyst; cost; design; design and construction; feeding; in vivo; innovation; method development; neoplastic cell; oxidation; planetary Atmosphere; pressure; programs; size; success; vapor

DESCRIPTION (provided by applicant): Low Cost Carbon Dioxide Generator for Cylinder-Free Cell Culture Incubators The proposed technology is a lower-cost method of controlling the gas atmosphere in cell culture incubators without the use of pressurized gas cylinders. The product would be an auxiliary unit/accessory to a cell culture incubator for laboratories that want the convenience and safety of no gas cylinder operation and the flexibility of dial-in oxygen and carbon dioxide set points over a wide range. The proposal specifically addresses a lower-cost chemical method of carbon dioxide generation for use in an integrated gas control system. Mammalian cell culture plays an important role in basic medical research, transplantation, tissue engineering, stem cell production, vaccine production, antibody production and cellular assays. Standard incubators for mammalian cell culture are typically operated at 142 mm Hg (18.6%) oxygen and 38 mm Hg (5%) carbon dioxide using pressured gas mixes. In vivo, the local concentration of both CO2 and O2 vary depending on the organ, cell position and disease states. The role of these gas concentrations in cellular physiology can be explored and then exploited with convenient, well-controlled cell culture incubators as proposed here. Lower concentrations of oxygen are required for research on stem cells, embryonic cells and tumor cells; higher concentrations may be advantageous for the culture of multilayer tissue such as skin, pancreatic islets and tissue engineering products. The 5% carbon dioxide set-point historically has arisen due to the common use of cell culture media with bicarbonate-based buffering; research on more physiologically-based carbon dioxide concentrations has been minimal to date. Giner, Inc.'s overall product goal is an auxiliary unit to commercial incubators to supply adjustable, controlled gas atmospheres (oxygen, carbon dioxide, nitrogen, humidity) using an electrochemical process to remove or add oxygen to the system and a chemical process to add carbon dioxide. The Phase I goal is to develop a low-cost method of generating carbon dioxide and to integrate a breadboard carbon dioxide generator with a cylinder-free gas control system. Commercial Potential: The cell culture incubator market is substantial and growing with over 16,000 carbon dioxide incubators sold in 2005 with $112 million in sales worldwide. The proposed technology would be a premium auxiliary/accessory unit for laboratories that want the convenience and safety of no gas cylinder operation and the flexibility of dial-in oxygen and carbon dioxide set points over a wide range. Giner will be partnering with a manufacturer and a distributor to commercialize the technology. RELEVANCE TO PUBLIC HEALTH: The proposed technology is a system to control the gas atmosphere in cell culture incubators. This will enhance basic research and commercial production that involves cell culture such as cancer research, transplantation, tissue engineering, stem cells, vaccine production, antibody production and cellular assays where specialized gas atmosphere control is required.

描述（由申请人提供）：用于无气瓶细胞培养孵化器的低成本二氧化碳发生器所提出的技术是一种在不使用加压气瓶的情况下控制细胞培养孵化器中的气氛的低成本方法。该产品将成为细胞培养箱的辅助装置/附件，适用于那些希望获得无气瓶操作的便利性和安全性以及在大范围内拨入氧气和二氧化碳设定点的灵活性的实验室。该提案特别提出了一种用于集成气体控制系统的低成本化学二氧化碳生成方法。哺乳动物细胞培养在基础医学研究、移植、组织工程、干细胞生产、疫苗生产、抗体生产和细胞测定中发挥着重要作用。用于哺乳动物细胞培养的标准培养箱通常使用加压气体混合物在 142 mm Hg (18.6%) 氧气和 38 mm Hg (5%) 二氧化碳下运行。在体内，CO2 和 O2 的局部浓度根据器官、细胞位置和疾病状态而变化。可以探索这些气体浓度在细胞生理学中的作用，然后使用此处提出的方便、控制良好的细胞培养箱进行利用。干细胞、胚胎细胞和肿瘤细胞的研究需要较低浓度的氧气；较高的浓度可能有利于多层组织的培养，例如皮肤、胰岛和组织工程产品。历史上 5% 二氧化碳设定点的出现是由于细胞培养基与碳酸氢盐缓冲液的普遍使用；迄今为止，对更多基于生理的二氧化碳浓度的研究还很少。 Giner, Inc. 的总体产品目标是商业孵化器的辅助装置，通过电化学过程向系统去除或添加氧气，并通过化学过程添加二氧化碳，提供可调节、受控的气体环境（氧气、二氧化碳、氮气、湿度）。第一阶段的目标是开发一种低成本的二氧化碳产生方法，并将面包板二氧化碳发生器与无气瓶气体控制系统集成。商业潜力：细胞培养箱市场规模巨大且不断增长，2005 年全球销售了 16,000 多台二氧化碳培养箱，销售额达 1.12 亿美元。对于那些希望获得无气瓶操作的便利性和安全性以及在大范围内拨入氧气和二氧化碳设定点的灵活性的实验室来说，所提出的技术将是一种优质的辅助/配件装置。 Giner 将与制造商和分销商合作，将该技术商业化。与公众健康的相关性：所提出的技术是一种控制细胞培养箱中气体氛围的系统。这将加强涉及细胞培养的基础研究和商业生产，例如癌症研究、移植、组织工程、干细胞、疫苗生产、抗体生产和需要专门气体气氛控制的细胞测定。