Portable Oxygen Gas Perfusion System for Pancreas Preservation

描述（由申请人提供）：拟议工作的目标是开发一种实质性改进的保存人类胰腺的技术，以提高从中收获的分离人类胰岛的质量，用于1型糖尿病的人类胰岛移植。目前，人体胰腺的运输采用两层储存方法（TLM）（8¿C），该方法使用含氧全氟碳溶液作为外部氧库。虽然TLM被广泛报道可以改善胰岛质量，但在获得可行的胰岛数量方面仍然存在不足。这部分是由于胰岛储存过程中活胰岛的损失。初步的计算和实验表明，胰腺的外部氧合只对器官外缘的组织有益，而胰腺的大部分体积没有氧合（85-95%）。这项提议的工作将使用一种新的氧气输送到器官内部的方法。研究和开发的另一个创新部分是开发一种系统，使用便携式电化学氧气浓缩器/发生器产生和调节氧气输送到器官。所提出的电化学氧气浓缩器利用电力从空气中浓缩氧气，并将其作为纯净的湿化氧气释放出来，这种氧气可以与周围空气混合，从而为所提出的保存技术提供最有效的pO2。将气态氧气输送到器官内部比器官的全液体灌注要简单。此外，拟议的系统将被设计为便携式，因为胰岛分离之前胰腺的主要“存储”时间是在将其运送到专门从胰岛中收获胰岛的设施期间。氧气将以所需的流量和压力连续产生，而不需要输送加压气瓶。这种提出的方法有可能在胰岛的质量上取得突破，而不是渐进式的改进。通过胰岛移植有效治疗重症1型糖尿病是前沿医疗保健研究的重点，本研究将有助于该领域的发展。该保存系统设备可以成为一种专业的商业产品，供应给主要的器官采集和移植中心。另外，利用所提出的技术，胰脏的运输和保存可以成为一种商业服务。该技术和设备可应用于其他器官的保存。关键词胰腺，胰腺，1型糖尿病，器官转运，胰岛移植，电化学氧浓度

DESCRIPTION (provided by applicant): The goal of the proposed work is to develop a substantially improved technique of preserving the human pancreas in order to improve the quality of the isolated human islets that are harvested from it for use in human islet transplantation for Type 1 diabetes. Currently, human pancreata are transported with the two layer method (TLM) of storage (8¿C), which uses an oxygenated perfluorocarbon solution as an external oxygen reservoir. While the TLM is widely reported to improve islet quality, there are still insufficiencies in the number of viable islets acquired. This is partially attributable to viable islet loss during pancreas storage. Preliminary calculations and experiments indicate that external oxygenation of the pancreas only benefits tissue in the outer rim of the organ leaving the majority of the volume of the pancreas without oxygenation (85-95%). The proposed work will use a novel method of oxygen delivery to the interior of the organ. Another innovative component of the research and development is to develop a system to generate and regulate oxygen delivery to the organ using a portable electrochemical oxygen concentrator/generator. The proposed electrochemical oxygen concentrator uses electrical power to concentrate oxygen from air and release it as pure, humidified oxygen that can be blended with ambient air to give the most efficacious pO2 for the proposed preservation technique. Delivery of gaseous oxygen to the interior of the organ is less complex than full liquid perfusion of the organ. Also the proposed system will be designed to be portable since the main "storage" time of the pancreas before islet isolation is during its transportation to a facility that specializes in harvesting islets from pancreata. Oxygen will be continuously generated at the desired flow rate and pressure without the need for transporting pressurized gas cylinders. This proposed method has the potential to effect a breakthrough, rather than an incremental improvement, in the quality of islets harvested from the pancreas. The effective treatment of serious cases of Type I Diabetes through pancreatic islet transplant is a high priority in cutting-edge healthcare research, and the proposed work will contribute to the advancement of the field. COMMERCIAL POTENTIAL The preservation system equipment could be a specialty commercial product, supplied to the major organ harvest and transplantation centers. Alternatively, the transportation and preservation of pancreata, using the proposed technique, could be a commercial service. The techniques and equipment could be applicable to the preservation of other organs. KEYWORDS pancreas, pancreata, Type I Diabetes, organ transport, islet transplantation, electrochemical oxygen concentration.