Cylinder-Free Atmosphere Control for Incubators

培养箱无气瓶气氛控制

• 批准号: 7260327
• 负责人: LINDA A TEMPELMAN
• 金额: $ 41.72万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7260327
• 关键词: Anodes; Antibody Formation; Area; Automation; Basic Science; Biomedical Research; Carbon Dioxide; Cell Count; Cell Line; Cell physiology; Cells; Cellular Assay; Collaborations; Computers; Construction Materials; Cultured Cells; Custom; Devices; Dimensions; Electronics; Environment; Equipment; Evaluation; Excision; Feedback; Gases; Generations; Goals; Government; Hand; Humidity; Incubators; Islets of Langerhans; Laboratories; Laboratory Research; Left; Life; Mammalian Cell; Manufacturer Name; Marketing; Measurement; Medical Research; Methanol; Microprocessor; Minnesota; Modification; Nitrogen; Operative Surgical Procedures; Oxygen; Pancreas; Performance; Persons; Phase; Play; Pliability; Plumbing; Preparation; Printing; Process; Production; Public Health; Purpose; Range; Regulation; Reporting; Research; Role; Running; Safety; Sales; Skin; Staging; Standards of Weights and Measures; Stem cells; System; Systems Integration; Technology; Temperature; Testing; Tissue Engineering; Tissues; Transplantation; Universities; Vaccine Production; anticancer research; blastomere structure; commercialization; cost; design; foot; improved; laptop; medical schools; neoplastic cell; oxidation; planetary Atmosphere; pressure; programs; prototype; research study; scale up; sensor; size

DESCRIPTION (provided by applicant): The proposed technology is a convenient and flexible system to control the gas atmosphere in cell culture incubators without the use of pressurized gas cylinders. The product would be an auxiliary unit/accessory to a cell culture incubator for laboratories that want the convenience and safety of no gas cylinder operation and the flexibility of dial-in oxygen and carbon dioxide set points over a wide range. Mammalian cell culture plays an important role in basic medical research, transplantation, tissue engineering, stem cell production, vaccine production, antibody production and cellular assays. Standard incubators for mammalian cell culture are typically operated at 142 mm Hg (18.6%) oxygen and 38 mm Hg (5%) carbon dioxide using pressured gas mixes. As the role of oxygen concentration in cellular physiology becomes more well understood, the need for adjustable oxygen incubators (1%-60%) for both research and production is becoming apparent. Lower concentrations of oxygen are required for research on stem cells, embryonic cells and tumor cells; higher concentrations may be advantageous for the culture of multilayer tissue such as skin, pancreatic islets and tissue engineering products. Giner, Inc.'s overall product goal is an auxiliary unit to commercial incubators to supply controlled gas atmospheres (oxygen, carbon dioxide, nitrogen, humidity) using electrochemical processes to generate all the gases required. In Phase I, feasibility was successfully demonstrated by providing gas generation and control to a small incubator with a prototype system. In Phase II, the technology will be sized for a range of commercial incubators, be packaged with an integrated electronic display and terminal and be more full- featured in preparation for commercialization. The Phase II prototype will be evaluated and utilized in a biomedical research laboratory at a medical school. Commercial Potential: The cell culture incubator market is substantial and growing with over 16,000 carbon dioxide incubators sold in 2005 with $112 million in sales worldwide. The proposed technology would be a premium auxiliary/accessory unit for laboratories that want the convenience and safety of no gas cylinder operation and the flexibility of dial-in oxygen and carbon dioxide set points over a wide range. Giner will be partnering with a manufacturer and a distributor to commercialize the technology. R+R Other #7 Project Narrative RELEVANCE TO PUBLIC HEALTH: The proposed technology is a system to control the gas atmosphere in cell culture incubators. This will enhance basic research and commercial production that involves cell culture such as cancer research, transplantation, tissue engineering, stem cells, vaccine production, antibody production and cellular assays where specialized gas atmosphere control is required.

描述（由申请人提供）：所提出的技术是一种方便且灵活的系统，可以在不使用加压气瓶的情况下控制细胞培养箱中的气体气氛。该产品将成为细胞培养箱的辅助装置/附件，适用于那些希望获得无气瓶操作的便利性和安全性以及在大范围内拨入氧气和二氧化碳设定点的灵活性的实验室。哺乳动物细胞培养在基础医学研究、移植、组织工程、干细胞生产、疫苗生产、抗体生产和细胞测定中发挥着重要作用。用于哺乳动物细胞培养的标准培养箱通常使用加压气体混合物在 142 mm Hg (18.6%) 氧气和 38 mm Hg (5%) 二氧化碳下运行。随着氧气浓度在细胞生理学中的作用得到越来越多的了解，研究和生产对可调节氧气培养箱 (1%-60%) 的需求变得越来越明显。干细胞、胚胎细胞和肿瘤细胞的研究需要较低浓度的氧气；较高的浓度可能有利于多层组织的培养，例如皮肤、胰岛和组织工程产品。 Giner, Inc. 的总体产品目标是商业孵化器的辅助装置，使用电化学过程提供受控气体环境（氧气、二氧化碳、氮气、湿度），以产生所需的所有气体。在第一阶段，通过为带有原型系统的小型培养箱提供气体发生和控制，成功证明了可行性。在第二阶段，该技术将适应一系列商业孵化器的规模，与集成电子显示器和终端打包在一起，并提供更全面的功能，为商业化做好准备。第二阶段原型将在医学院的生物医学研究实验室进行评估和使用。商业潜力：细胞培养箱市场规模巨大且不断增长，2005 年全球销售了 16,000 多台二氧化碳培养箱，销售额达 1.12 亿美元。对于那些希望获得无气瓶操作的便利性和安全性以及在大范围内拨入氧气和二氧化碳设定点的灵活性的实验室来说，所提出的技术将是一种优质的辅助/配件装置。 Giner 将与制造商和分销商合作，将该技术商业化。 R+R 其他#7 项目叙述与公众健康的相关性：所提议的技术是一种控制细胞培养箱中气体氛围的系统。这将加强涉及细胞培养的基础研究和商业生产，例如癌症研究、移植、组织工程、干细胞、疫苗生产、抗体生产和需要专门气体气氛控制的细胞测定。