Portable Oxygen Gas Perfusion System for Pancreas Preservation

用于保存胰腺的便携式氧气灌注系统

• 批准号: 7811845
• 负责人: LINDA A TEMPELMAN
• 金额: $ 8.91万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7811845
• 关键词: Air; Anodes; Biological Preservation; Cathodes; Clinical; Complex; Computer Systems Development; Equipment; Failure; Family suidae; Fluorocarbons; Gases; Generations; Goals; Harvest; Health Services Research; Heart; Human; In Situ; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Kidney; Laboratories; Left; Liquid substance; Literature; Liver; Methods; Minnesota; Organ; Organ Harvestings; Organ Transplantation; Oxygen; Pancreas; Perfusion; Phase; Preservation Technique; Relative (related person); Reporting; Research; Research Personnel; Rewarming; Services; Site; Solutions; Stream; System; Techniques; Time; Tissues; Transplantation; Transportation; Universities; Wisconsin; Work; design; effective therapy; improved; innovation; islet; medical specialties; novel; pressure; programs; research and development; research study; success; transplantation typing

DESCRIPTION (provided by applicant): The goal of the proposed work is to develop a substantially improved technique of preserving the human pancreas in order to improve the quality of the isolated human islets that are harvested from it for use in human islet transplantation for Type 1 diabetes. Currently, human pancreata are transported with the two layer method (TLM) of storage (8¿C), which uses an oxygenated perfluorocarbon solution as an external oxygen reservoir. While the TLM is widely reported to improve islet quality, there are still insufficiencies in the number of viable islets acquired. This is partially attributable to viable islet loss during pancreas storage. Preliminary calculations and experiments indicate that external oxygenation of the pancreas only benefits tissue in the outer rim of the organ leaving the majority of the volume of the pancreas without oxygenation (85-95%). The proposed work will use a novel method of oxygen delivery to the interior of the organ. Another innovative component of the research and development is to develop a system to generate and regulate oxygen delivery to the organ using a portable electrochemical oxygen concentrator/generator. The proposed electrochemical oxygen concentrator uses electrical power to concentrate oxygen from air and release it as pure, humidified oxygen that can be blended with ambient air to give the most efficacious pO2 for the proposed preservation technique. Delivery of gaseous oxygen to the interior of the organ is less complex than full liquid perfusion of the organ. Also the proposed system will be designed to be portable since the main "storage" time of the pancreas before islet isolation is during its transportation to a facility that specializes in harvesting islets from pancreata. Oxygen will be continuously generated at the desired flow rate and pressure without the need for transporting pressurized gas cylinders. This proposed method has the potential to effect a breakthrough, rather than an incremental improvement, in the quality of islets harvested from the pancreas. The effective treatment of serious cases of Type I Diabetes through pancreatic islet transplant is a high priority in cutting-edge healthcare research, and the proposed work will contribute to the advancement of the field. COMMERCIAL POTENTIAL The preservation system equipment could be a specialty commercial product, supplied to the major organ harvest and transplantation centers. Alternatively, the transportation and preservation of pancreata, using the proposed technique, could be a commercial service. The techniques and equipment could be applicable to the preservation of other organs. KEYWORDS pancreas, pancreata, Type I Diabetes, organ transport, islet transplantation, electrochemical oxygen concentration.

描述（由适用提供）：拟议工作的目的是开发一种维护人类胰腺的大大改进的技术，以提高从中收获的孤立人类胰岛的质量，用于用于1型糖尿病的人类胰岛移植中。目前，人胰腺是使用储存的两层方法（TLM）（8¿C）运输的，该方法使用氧化的全氟碳溶液作为外氧晶状体。尽管TLM被广泛报道以提高胰岛质量，但获取的可行胰岛数量仍然不足。这部分归因于胰腺存储期间可行的胰岛损失。初步计算和实验表明，胰腺的外充氧仅使器官外边缘的组织有益于使胰腺体积的大部分没有氧合（85-95％）。拟议的工作将使用新颖的研究和开发的另一个创新组成部分，是开发一种系统，使用便携式电化学氧浓度/发电机来生成和调节向器官的氧气递送。提出的电化学氧浓缩物使用电力从空气中浓缩氧气，并将其释放为纯净的，加湿的氧气，可以与环境空气混合，为提出的保存技术提供最有效的PO2。与器官的全液体灌注相比，向器官内部递送气体氧。另外，提议的系统将设计为可移植，因为胰岛隔离之前的主要“存储”时间是在运输到专门从胰腺收获胰岛的设施中。氧气将在所需的流量和压力下连续产生，而无需运输加压气缸。这种提出的方​​法有可能在胰腺收获的胰岛的质量上实现突破而不是增量的改进。通过胰岛移植对严重的I型糖尿病病例的有效治疗是最先进的医疗研究的优先事项，拟议的工作将有助于该领域的发展。商业潜能制备系统设备可能是一种特殊的商业产品，可提供给主要的器官收获和移植中心。另外，使用拟议技术的胰腺运输和保存可能是商业服务。该技术和设备可用于保存其他器官。关键字胰腺，胰腺，I型糖尿病，器官传输，胰岛移植，电化学氧浓度。

项目成果

Continuous real-time viability assessment of kidneys based on oxygen consumption.

• DOI: 10.1016/j.transproceed.2010.05.082
• 发表时间: 2010-07
• 期刊: TRANSPLANTATION PROCEEDINGS
• 影响因子: 0.9
• 作者: Weegman, B. P.;Kirchner, V. A.;Scott, W. E., III;Avgoustiniatos, E. S.;Suszynski, T. M.;Ferrer-Fabrega, J.;Rizzari, M. D.;Kidder, L. S.;Kandaswamy, R.;Sutherland, D. E. R.;Papas, K. K.
• 通讯作者: Papas, K. K.