NONINVASIVE DETECTION OF NEW SIV LINEAGES IN CAPTIVE SOOTY MANGABEYS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。建立了一种无创检测人工饲养黑白眉（Cercocebus atys）粪便中SIVsm病毒粒子RNA的方法。利用该方法研究野生濒危SMs中SIVsm的自然历史，有助于了解SIVsm与HIV-2的多样性和进化。对61只野生短尾猿和14只西非野生黑猩猩的粪便进行了研究。1993年在塞拉利昂农村采集了样本。1例SM样品经逆转录- pcr检测呈阳性。在14只黑猩猩的粪便中没有检测到病毒序列。从sm# 13中获得的env序列的系统发育分析表明，它聚集在SIVsm群中，包括SIVsmH4， B670和PBj，确定了以前未知的SIV与西非和美国SM菌落的联系。该病毒被命名为SIVsmSL93g，表明SIVB670-SIVsmH4-SIVPbj集群起源于塞拉利昂北部。该菌株在野生SM中的发现也为野外采集猴粪进行SIV检测提供了一种无创方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed a noninvasive method for SIVsm virion RNA detection in feces of captive sooty mangabeys (SMs) (Cercocebus atys). Employing this method to investigate the natural history of SIVsm in endangered SMs in the wild is useful for understanding diversity and evolution of SIVsm and HIV-2. Fecal samples collected from 61 wild SMs and 14 wild west African chimpanzees (Pan troglodytes verus) were studied. Samples were collected in rural Sierra Leone in 1993. One SM sample tested positive by reverse transcriptase-PCR. No viral sequences were detected in 14 feces of chimpanzees. Phylogenetic analysis of env sequence obtained from SM #13 showed that it clustered within the SIVsm group that includes SIVsmH4, B670 and PBj, identifying a previously unknown SIV connection from West Africa and American based colonies of SM. The virus, designated as SIVsmSL93g, indicates that the SIVB670-SIVsmH4-SIVPbj cluster originated in Northern Sierra Leone. The discovery of this strain in wild SM also indicates that noninvasive methods can be used for SIV detection from monkey feces collected in the field.