ELICITATION OF BROAD IMMUNITY USING VLPS WITH CONSENSUS ENVS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 从2009年5月至2009年9月对动物进行采样，作为2009年3月病毒挑战的后续行动。标本用于监测免疫反应、血浆病毒载量(PVL)。 2009年9月，对原16只动物中的10只进行了4次抗CD8抗体处理。在抗CD8治疗之前，所有10只动物都有无法检测到的PVL(4只动物从未表现出可检测到的病毒载量，6只动物在攻击后病毒载量呈阳性，但至少在治疗前21天无法检测到)。2009年11月采集了血液样本，以监测CD8水平、免疫反应和血浆病毒载量。治疗后，所有动物的CD8水平显著下降，随后CD8细胞缓慢恢复。其中5只动物的PVL在治疗后仍低于可检测到的限度。 其中五只动物在抗CD8治疗后表现出血浆病毒载量的回升(所有动物在挑战后都表现出可检测到的病毒载量)。在抗CD8治疗后的30天内，这5只动物的PVL回到了可检测到的限度以下。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Animals were sampled from May 2009  September 2009 as follow-up from viral challenge in March 2009. Samples were used to monitor immune response, plasma viral loads (PVL). In September 2009, ten of the original 16 animals were treated four times with anti-CD8 antibody. Prior to the anti-CD8 treatment, all ten animals had undetectable PVL (four animals never exhibited detectable viral loads, six animals had positive viral loads after challenge but were undetectable at least 21 days prior to treatment). Blood samples were collected through November 2009 to monitor CD8 levels, immune response and plasma viral loads. After treatment, all animals showed significant drops in CD8 levels followed by a slow recovery of CD8 cells. The PVL of five of the treated animals remained below detectable limits after treatment. Five of the animals exhibited a resurgence of plasma viral loads after anti-CD8 treatment (all had previously exhibited detectable viral loads after challenge). Within 30 days after anti-CD8 treatment, the PVL of these five animals returned to below detectable limits.