CORE--Analytical

核心——分析

• 批准号: 7106962
• 负责人: Keri C Hornbuckle
• 金额: $ 39.54万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7106962
• 关键词: analytical method; biomedical facility; chemical structure; chemical synthesis; data management; environmental exposure; halobiphenyl /halotriphenyl compound; hydroxylation; isotope dilution method; method development; training

The Analytical Core provides routine and non-routine analytical service to the University of Iowa research program. The core will provide expert staff, equipment, and instrumentation in line with five Aims. Specific Aim 1 is the development of analytical and quality control/assurance standards. In close collaboration with the Synthesis Core, which is responsible for acquiring and archiving individual compound standards and preparing stock solutions, the Analytical Core will develop the mathematical methods for congener representation, analyze single congener solutions for purity assessment and quality control, and test the prepared standard solutions against certified Standard Reference Materials, including PCB calibration mixtures. Specific Aim 2 is the development of a mixture of PCB congeners for laboratory animal exposure studies. The Analytical Core will develop the mathematical methods to design the exposure mixture and test the prepared mixture against certified Standard Reference Materials. Specific Aim 3 is the analysis of environmental and laboratory samples for a suite of PCB congeners (>120). The Analytical Core will extract and analyze PCBs on a congener-specific basis. Applying the primary calibration standard developed under Aim 1, samples will be quantified by the isotope dilution method and the internal standard method. Internal Standards and .Surrogate Recovery Standards developed under Aim 1 will be used to precisely determine PCB concentrations in samples. Methods for analysis on a congener-specific basis will be assessed using performance standard injections and analysis of certified Standard Reference Materials. Some analyses will be performed on the entire suite of PCB congeners, including laboratory animals exposed to the mixture described in Aim 2 and including blood from humans and non-laboratory animals. Other samples will be analyzed for specific congeners and metabolites in support of investigators focused on congener-specific toxicology. Specific Aim 4 involves the extraction, identification, and quantification of unknown metabolites. The Analytical Core will determine purity of synthesized metabolites and associated analytical standards, then analyze for hydroxylated PCBs and other PCB metabolites. As requested by the sbrp investigators, the Analytical Core will also train personnel and assist in sampling design and implementation. Specific Aim 5 is the design and maintenance of a database system. A redundant storage architecture with automatic backup and password protection will be used to allow investigators to download data and reports produced by the Analytical Core.

分析核心为爱荷华州大学的研究提供常规和非常规分析服务 程序.核心将提供专家人员，设备和仪器，符合五个目标。具体目标1 是制定分析和质量控制/保证标准。密切配合下 合成核心，负责获取和存档单个化合物标准品，并准备 储备溶液，分析核心将开发同源物表示的数学方法，分析 用于纯度评估和质量控制的单一同系物溶液，并检测制备的标准品溶液 对照经认证的标准参考物质，包括多氯联苯校准混合物。具体目标2是 开发多氯联苯同系物混合物，用于实验室动物接触研究。分析核心 将开发数学方法来设计曝光混合物，并测试制备的混合物， 经认证的标准参考物质。具体目标3是环境和实验室分析 一套多氯联苯同系物的样本（>120）。分析核心将提取和分析多氯联苯在一个 同类特异性基础。应用根据目标1开发的一级校准标准品， 通过同位素稀释法和内标法定量。内标物和替代物 根据目标1制定的回收标准将用于精确测定样品中的多氯联苯浓度。 将使用性能标准品进样和 分析认证的标准参考物质。将对整套PCB进行一些分析 同系物，包括接触目标2所述混合物的实验室动物， 人类和非实验室动物。其他样本将分析特定的同系物和代谢物， 对研究人员的支持侧重于同类物特定毒理学。具体目标4涉及提取， 未知代谢物的鉴定和定量。分析核心将确定 合成代谢物和相关分析标准品，然后分析羟基化多氯联苯和其他多氯联苯 代谢物。根据SBRP调查人员的要求，分析核心还将培训人员并协助 抽样设计和实施。具体目标5是数据库系统的设计和维护。一 将使用具有自动备份和密码保护的冗余存储架构， 下载分析核心产生的数据和报告。