Research Support Core: Analytical Core

研究支持核心：分析核心

• 批准号: 10559670
• 负责人: Keri C Hornbuckle
• 金额: $ 25.99万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10559670
• 关键词: Agreement; Archives; Carcinogens; Collaborations; Complex; Core Facility; Data; Data Analyses; Databases; Detection; Development; Environment; FAIR principles; Faculty; Food; Food Analysis; Foundations; Frequencies; Health Hazards; Human; Hydroxylation; Infrastructure; Iowa; Laboratories; Laboratory Animals; Learning Module; Measurement; Metabolic; Methods; Modeling; Neurologic; Paint; Peer Review; Pigments; Plant Resins; Plants; Polychlorinated Biphenyls; Polymers; Postdoctoral Fellow; Procedures; Protocols documentation; Publications; Quality Control; Quartz; Recovery; Reference Standards; Reproducibility; Research; Research Personnel; Research Support; Risk; Sampling; Serum; Services; Signal Recognition Particle; Soil; Source; Standardization; Superfund; Supervision; Technology; Testing; Tissues; Toxic effect; Training; Update; Water; air sampling; animal tissue; data integration; data management; data sharing; experience; graduate student; high throughput analysis; innovation; instrument; instrumentation; method development; microbial; polyurethane foam; programs; quality assurance; remediation; sharing platform; statistics; superfund chemical; water sampling

PROJECT SUMMARY: Analytical Core Polychlorinated biphenyls (PCBs) are important Superfund chemicals that are known human carcinogens also associated with neurological and metabolic disruption. The Analytical Core (AC) provides analytical services to the ISRP to facilitate high-quality research on PCBs, including data, standard operating procedures, and peer- reviewed publications that are standardized, efficient, and comparable across all ISRP matrices and projects. The AC provides instrumentation and facilities, expert staff, training, data sharing platforms, and support for method development. The AC will continue to accomplish these activities through five Specific Aims: Aim 1. Maintain analytical quality assurance (QA) standards and protocols. Accurate, precise, representative, reproducible, and comparable sample analysis is absolutely essential for all five ISRP projects and is a key AC objective. The exceptional rigor of the QA protocol for PCB analysis provides a critical foundation for discoveries by the ISRP projects. Aim 2. Facilitate high-throughput analysis in complex biotic and abiotic matrices. The AC supports ISRP projects by extracting and analyzing samples for all 209 PCBs and 72 OH- PCBs as MeO-PCBs in complex matrices, including: air samples collected on quartz filters, XAD resin, and polyurethane foam; sediment, water, and pore water samples; human serum; laboratory animal tissues; plant tissues; soils; microbial cultures; and polymers, paints, and pigments. To support new research directions within the ISRP, we will expand our capabilities to include analyses of food matrices and the ability to quantify all 837 possible mono-hydroxylated PCBs. Aim 3. Provide prioritized training and access to AC facilities. The AC, in collaboration with the Research Experience and Training Coordination Core, provides prioritized, standardized training in AC facilities and methods to graduate student and postdoc trainees, faculty, and staff from all ISRP projects. Trainees also receive assistance in method development and use of instrumentation. Aim 4. Support method development for complex matrices. The AC, in collaboration with ISRP projects, will continue to develop, maintain, and update methods for high-throughput PCB and OH-PCB extraction and analysis in biotic and abiotic samples. Separate but comparable methods are maintained across matrices to facilitate integrated data analysis. Aim 5. Maintain analytical infrastructure. The AC maintains critical infrastructure, including high-use analytical instruments, a sample database, and archives such as standard solutions, samples, and sample extracts. The AC, in collaboration with the Data Management and Analysis Core, will provide oversight to make data findable, accessible, interoperable, and reusable (FAIR) by ISRP investigators. Overall, the AC is a fully-integrated support core that assists ISRP projects to meet all four SRP mandates requiring methods for identifying human health hazards of superfund chemicals, detection, assessment of exposure risk, and remediation.

项目总结：分析核心 多氯联苯（PCBs）是重要的超级基金化学品，也是已知的人类致癌物 与神经和代谢紊乱有关分析核心（AC）提供分析服务， ISRP促进对PCB的高质量研究，包括数据，标准操作程序和同行， 审查了标准化、高效且在所有ISRP矩阵和项目中具有可比性的出版物。 AC提供仪器和设施，专家人员，培训，数据共享平台，并支持 方法开发咨询委员会将继续通过五个具体目标完成这些活动：目标1。 维护分析质量保证（QA）标准和方案。准确，精确，有代表性， 对于所有五个ISRP项目来说，重复性和可比性的样品分析是绝对必要的，也是关键的AC objective. PCB分析的QA协议的异常严格性为以下方面提供了关键基础： ISRP项目的发现。目标2.促进复杂生物和非生物环境中的高通量分析 矩阵AC支持ISRP项目，提取和分析所有209种多氯联苯和72种OH-的样品。 复杂基质中的MeO-PCBs，包括：在石英过滤器、XAD树脂上收集的空气样本， 聚氨酯泡沫;沉积物、水和孔隙水样品;人血清;实验动物组织;植物 组织;土壤;微生物培养物;以及聚合物、油漆和颜料。支持新的研究方向 在ISRP中，我们将扩大我们的能力，包括食品基质分析和量化能力， 所有837种可能的单羟基多氯联苯目标3。提供优先培训和使用空调设施的机会。的 AC与研究经验和培训协调核心合作，提供优先， 对研究生和博士后学员、教职员工进行空调设施和方法的标准化培训 所有ISRP项目。受训人员还在方法开发和仪器使用方面得到帮助。 目标4。支持复杂矩阵的方法开发。AC将与ISRP项目合作， 继续开发、维护和更新高通量PCB和OH-PCB提取方法， 生物和非生物样品的分析。在不同基质中保留单独但相当的方法， 促进综合数据分析。目标5。维护分析基础设施。AC保持关键 基础设施，包括高使用率的分析仪器、样品数据库和标准 溶液、样品和样品浸提液。AC与数据管理和分析部门合作， 核心，将提供监督，使ISRP的数据可查找，可访问，可互操作和可重用（FAIR） investigators.总体而言，AC是一个完全集成的支持核心，可协助ISRP项目满足所有四个SRP 要求确定超级基金化学品对人类健康危害的方法，检测， 暴露风险评估和补救措施。