Analytical Core: Extraction, Detection, and Interpretation of PCB Congeners in Cmplex Matrices

分析核心：复杂矩阵中 PCB 同系物的提取、检测和解释

• 批准号: 8919610
• 负责人: Keri C Hornbuckle
• 金额: $ 24.77万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8919610
• 关键词: Address; Adult; Agreement; Air; Animals; Archives; Area; Basic Science; Biological; Body Burden; Breathing; Calibration; Characteristics; Chemicals; Child; Complex; Databases; Detection; Development; Electronics; Environment; Environmental Pollutants; Environmental sludge; Foundations; Frequencies; Furniture; Goals; Health; Human; Human Resources; Hydroxyl Radical; Individual; Injection of therapeutic agent; Inorganic Sulfates; Institutes; Iowa; Journals; Laboratories; Laboratory Animals; Link; Maintenance; Measurement; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Mus; Paint; Parents; Peer Review; Plant Resins; Plants; Policies; Polychlorinated Biphenyls; Procedures; Process; Program Research Project Grants; Property; Protocols documentation; Publishing; Quality Control; Quartz; Rattus; Recommendation; Recording of previous events; Reference Standards; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resistance; Sampling; Schools; Serum; Services; Shipping; Ships; Signal Transduction; Soil; Solutions; Source; Sunlight; Superfund; Technology; Time; Tissues; Toxic effect; Training; United States Environmental Protection Agency; Universities; Unspecified or Sulfate Ion Sulfates; Urine; air sampling; animal tissue; building materials; community based participatory research; data sharing; design; high throughput analysis; innovation; instrument; instrumentation; manufacturing process; meetings; method development; photolysis; pollutant; polyurethane foam; programs; quality assurance; repaired; response; signal processing; tandem mass spectrometry; water sampling

PROJECT SUMMARY PCB analysis for research purposes is labor-intensive, requires specialized instrumentation, and depends on excellent quality control procedures. Because the Analytical Core provides the trained staff, maintains the instruments and facilities, and provides methods to assure accuracy, precision, representativeness, comparability, and reproducibility, the isrp research projects are able to make high impact discoveries, publish their results in peer-review journals, and more quickly provide scientific guidance regarding PCBs in the environment and their effect on human health. The Analytical Core supports the research of the isrp by extracting and analyzing samples for all 209 PCBs and for individual compounds in complex matrices including air samples; harbor sediment; human blood serum; urine; laboratory animal tissues; plant tissues; soils; sludge; laboratory solutions, building materials, paint, and furniture. Each year the Analytical Core will complete thousands of such measurements as well as hundreds of quality control measurements and method development measurements. The Analytical Core provides detailed quality control guidance and collaborates closely with isrp researchers to determine appropriate methods for quality assurance control. Analyses for PCB congeners in environmental matrices includes routine use of surrogate standards injected in every sample to assess and correct for analytical efficiency; internal standards for quantification; duplicate injections; laboratory blanks, calibration standards, and frequent analysis of materials with certified concentrations of PCBs. The rigor of the QC/QA protocol is unusual for PCB analysis but provides the critical foundation for discoveries in the research projects. The Analytical Core has a sustained history of innovation, starting with the development of tandem mass spectrometry for congener-specific analysis of PCBs in environmental and laboratory samples. This method enabled many important discoveries and innovative findings in the isrp, including the ability to link specific toxic properties by compound; accurate determination of the lower molecular weight compounds; and precise measurement of all PCBs. The congener-specific method used by the Analytical Core enabled the discovery of non-Aroclors in the environment. The study of these compounds, which are currently produced through some chemical manufacturing processes, has been a major area of focus since that time. Innovations planned include the development of efficient methods for routine analysis of known and as-yet unknown decay products of PCBs. Although PCBs are very persistent and resistant to decay, many PCBs can be broken down by sunlight and by metabolism in plants, animals and humans. The Analytical Core assists the research projects in measuring those decay products. .

项目摘要 用于研究目的的PCB分析是劳动密集型的，需要专门的仪器，并依赖于 优秀的质量控制程序。由于分析核心提供训练有素的工作人员， 仪器和设施，并提供方法，以确保准确性，精密度，代表性， 可比较性和可重复性，isrp的研究项目能够做出高影响力的发现， 他们的研究结果发表在同行评审期刊上，并更快地提供有关多氯联苯的科学指导， 环境及其对人类健康的影响。 分析核心通过提取和分析所有209种多氯联苯的样品来支持isrp的研究 以及复杂基质中的单个化合物，包括空气样本、港口沉积物、人体血液 血清;尿液;实验室动物组织;植物组织;土壤;污泥;实验室溶液，建筑材料， 油漆和家具。每年，分析核心将完成数千次这样的测量， 数百项质量控制测量和方法开发测量。分析核心 提供详细的质量控制指导，并与isrp研究人员密切合作， 质量保证控制的适当方法。环境基质中多氯联苯同系物的分析 包括在每份样品中注入替代标准品的常规使用，以评估和校正分析 效率;定量用内标;重复进样;实验室空白，校准标准品， 并经常分析含有经认证的多氯联苯浓度的材料。QC/QA协议的严格性是 这对于PCB分析来说是不寻常的，但为研究项目中的发现提供了关键基础。 分析核心有一个持续的创新历史，从串联质量的发展开始 用于环境和实验室样品中多氯联苯同系物特定分析的光谱法。该方法 使许多重要的发现和创新性的发现，在isrp，包括能够连接特定的有毒物质， 性能的化合物;准确测定较低分子量的化合物;和精密 测量所有PCB。分析核心使用的同源物特异性方法使得能够发现 环境中的非Aroclors。这些化合物的研究，目前是通过一些 自那时以来，化学制造工艺一直是重点关注的主要领域。计划的创新 包括开发有效的方法，对已知和未知的衰变产物进行常规分析 PCBs。虽然多氯联苯具有很强的持久性和抗腐蚀性，但许多多氯联苯可被 通过植物、动物和人类的新陈代谢。分析核心协助研究项目 来测量这些衰变产物。 .