Analytical Core: Extraction, Detection, and Interpretation of PCB Congeners in Cmplex Matrices

分析核心：复杂矩阵中 PCB 同系物的提取、检测和解释

• 批准号: 9149263
• 负责人: Keri C Hornbuckle
• 金额: $ 25.74万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9149263
• 关键词: Address; Adult; Agreement; Air; Animals; Archives; Area; Basic Science; Biological; Body Burden; Breathing; Calibration; Characteristics; Chemicals; Child; Complex; Databases; Detection; Development; Electronics; Environment; Environmental Pollutants; Environmental sludge; Foundations; Frequencies; Furniture; Goals; Health; Human; Human Resources; Hydroxyl Radical; Individual; Injection of therapeutic agent; Inorganic Sulfates; Institutes; Iowa; Journals; Laboratories; Laboratory Animals; Link; Maintenance; Measurement; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Mus; Paint; Parents; Peer Review; Plant Resins; Plants; Policies; Polychlorinated Biphenyls; Procedures; Process; Program Research Project Grants; Property; Protocols documentation; Publishing; Quality Control; Quartz; Rattus; Recommendation; Recording of previous events; Reference Standards; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resistance; Sampling; Schools; Serum; Services; Shipping; Ships; Signal Transduction; Soil; Source; Sunlight; Superfund; Technology; Time; Tissues; Toxic effect; Training; United States Environmental Protection Agency; Universities; Unspecified or Sulfate Ion Sulfates; Urine; air sampling; animal tissue; building materials; community based participatory research; data sharing; design; high throughput analysis; innovation; instrument; instrumentation; manufacturing process; meetings; method development; photolysis; pollutant; polyurethane foam; programs; quality assurance; repaired; response; signal processing; tandem mass spectrometry; water sampling

PROJECT SUMMARY PCB analysis for research purposes is labor-intensive, requires specialized instrumentation, and depends on excellent quality control procedures. Because the Analytical Core provides the trained staff, maintains the instruments and facilities, and provides methods to assure accuracy, precision, representativeness, comparability, and reproducibility, the isrp research projects are able to make high impact discoveries, publish their results in peer-review journals, and more quickly provide scientific guidance regarding PCBs in the environment and their effect on human health. The Analytical Core supports the research of the isrp by extracting and analyzing samples for all 209 PCBs and for individual compounds in complex matrices including air samples; harbor sediment; human blood serum; urine; laboratory animal tissues; plant tissues; soils; sludge; laboratory solutions, building materials, paint, and furniture. Each year the Analytical Core will complete thousands of such measurements as well as hundreds of quality control measurements and method development measurements. The Analytical Core provides detailed quality control guidance and collaborates closely with isrp researchers to determine appropriate methods for quality assurance control. Analyses for PCB congeners in environmental matrices includes routine use of surrogate standards injected in every sample to assess and correct for analytical efficiency; internal standards for quantification; duplicate injections; laboratory blanks, calibration standards, and frequent analysis of materials with certified concentrations of PCBs. The rigor of the QC/QA protocol is unusual for PCB analysis but provides the critical foundation for discoveries in the research projects. The Analytical Core has a sustained history of innovation, starting with the development of tandem mass spectrometry for congener-specific analysis of PCBs in environmental and laboratory samples. This method enabled many important discoveries and innovative findings in the isrp, including the ability to link specific toxic properties by compound; accurate determination of the lower molecular weight compounds; and precise measurement of all PCBs. The congener-specific method used by the Analytical Core enabled the discovery of non-Aroclors in the environment. The study of these compounds, which are currently produced through some chemical manufacturing processes, has been a major area of focus since that time. Innovations planned include the development of efficient methods for routine analysis of known and as-yet unknown decay products of PCBs. Although PCBs are very persistent and resistant to decay, many PCBs can be broken down by sunlight and by metabolism in plants, animals and humans. The Analytical Core assists the research projects in measuring those decay products. .

项目总结 用于研究目的的印刷电路板分析是劳动密集型的，需要专门的仪器，并依赖于 卓越的质量控制程序。因为分析核心提供训练有素的工作人员，维护 仪器和设施，并提供方法，以确保准确性，精密度，代表性， 可比性和可重复性，ISRP研究项目能够做出影响很大的发现，发布 他们的结果发表在同行评议期刊上，并更快地提供关于 环境及其对人类健康的影响。 分析核心通过提取和分析所有209个多氯联苯的样品来支持ISRP的研究 以及复杂基质中的单个化合物，包括空气样本、港口沉积物和人体血液 血清；尿液；实验动物组织；植物组织；土壤；污泥；实验室溶液，建筑材料， 油漆和家具。每年分析核心将完成数千次这样的测量以及 数百项质量控制措施和方法开发措施。分析的核心 提供详细的质量控制指导，并与ISRP研究人员密切合作，以确定 适当的质量保证控制方法。环境基质中多氯联苯同系物的分析 包括常规使用在每个样本中注入的替代标准，以评估和更正分析 效率；定量的内部标准；重复注射；实验室空白，校准标准， 以及频繁分析经认证的多氯联苯浓度的材料。QC/QA协议的严格性是 对于印刷电路板分析来说是不寻常的，但为研究项目中的发现提供了关键的基础。 分析核心具有持续的创新历史，从串联质量的发展开始 用于环境和实验室样品中多氯联苯的同种物质特定分析的光谱分析。这种方法 在ISRP中实现了许多重要的发现和创新发现，包括将特定的有毒物质 化合物的性质；低分子化合物的准确测定；以及精确 所有多氯联苯的测量。分析核心使用的特定于同类的方法使发现 环境中的非Aroclors。对这些化合物的研究，目前是通过一些 从那时起，化学制造工艺一直是一个主要的关注领域。计划中的创新 包括开发对已知和未知衰变产物进行常规分析的有效方法 多氯联苯。尽管多氯联苯具有很强的持久性和耐腐蚀性，但许多多氯联苯可以通过 阳光以及植物、动物和人类的新陈代谢。分析核心协助研究项目 来测量那些腐烂的产物。 。