Modulation of Gene Expression Through RNAi

在过去的四年里，该计划项目越来越多地使用复杂的基因 探索基因功能的策略。该产品组合的一个关键组成部分是全基因组RNAi资源，该资源 可用于探索单个基因的功能或对这些基因进行全基因组搜索 这影响了一个感兴趣的过程。核心不仅管理该计划的大规模RNAi库，而且 为它们的使用提供所有必要的支持。这一核心的八大功能如下。 1.大规模shRNA载体文库的筛选 2.向计划成员分发单个克隆和克隆集 3.基于RNA的抑制剂的设计 4.RNAi工具的质量控制 5.定制RNAi载体的构建 6.支持重组逆转录病毒的生产 7.组织培养试剂的质量控制 8.将试剂存档并分发给社区 通过这些服务，核心为本计划内的每个项目提供支持，并发挥 在将CSHL开发的RNAi试剂提供给更广泛的科学界方面发挥重要作用。 该核心为管理全基因组RNAi文库提供了唯一的赠款支持，这些RNAi文库具有 通过本计划的工作直接广泛传播。

Over its past four years, the Program Project has made ever-increasing use of sophisticated genetic strategies for probing gene function. A key component of the portfolio is a genome-wide RNAi resource that can be used to probe the function of individual genes or to carry out genome-wide searches for those loci that impact a process of interest. The core not only manages the Program's large-scale RNAi libraries but provides all necessary support for their use. The eight major functions of this core are as follows. 1. Curation of large scale shRNA vector libraries 2. Distribution of individual clones and clone sets to Program members 3. Design of RNA-based inhibitors 4. Quality control of RNAi tools 5. Construction of custom RNAi vectors 6. Support production of recombinant retroviruses 7. Quality control of tissue culture reagents 8. Archiving and distribution of reagents to the community Through these services, the Core provides support to every Project within this Program and also plays an essential role in making the RNAi reagents developed at CSHL available to the broader scientific community. This Core provides the only grant support for the management of the genome-wide RNAi libraries that have been widely disseminated directly through the work of this Program.