LC/MDS TOF SPECTROMETER FOR INSTRUMENT FACILITY AT CSU: CHEMISTRY

用于科罗拉多州立大学仪器设施的 LC/MDS TOF 光谱仪：化学

• 批准号: 7335266
• 负责人: Robert Michael Williams
• 金额: $ 26.05万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335266
• 关键词: adduct; fluid; gait; high performance liquid chromatography; ions; mass spectrometry; measurement; motivation; quality of life; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Chemistry Department at Colorado State University is requesting funds to purchase a new time-of-flight mass spectrometer (LC/MSD TOF) equipped with a high performance liquid chromatography (HPLC) system. The instrument will be managed within the Department's Central Instrumentation Facility (GIF). At the present time, we do the greatest portion of our mass spectrometry work on two heavily used instruments, one of which is well past its prime. The newer instrument (a ThermoElectron LCQ Duo interfaced to an older Hewlett-Packard 1100 HPLC) operates in our open-access laboratory and is absolutely saturated with users. The other instrument is a thirteen year old Fisons VG AutoSpec set up to perform nominal and accurate mass measurements mostly by Liquid Secondary Ion MS. The new LC/MSD TOF will address the need for more HPLC/MS capacity as well as more accurate mass measurement capacity. We anticipate that for certain routine samples our throughput will increase by three to five times, maybe even more. Most importantly, the process can be automated and set to run during periods of lower instrument demand. The new LC/MSD TOF system will also allow us to measure all ions of interest (impurities, various adducts and fragments) to the required accuracy in only one operation. Finally, many of these measurements will be made in an open access environment.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：科罗拉多州立大学化学系正在申请资金购买配备高效液相色谱 (HPLC) 系统的新型飞行时间质谱仪 (LC/MSD TOF)。该仪器将在该部门的中央仪器设施（GIF）内进行管理。目前，我们的大部分质谱工作是在两台频繁使用的仪器上完成的，其中一台已经过了鼎盛时期。较新的仪器（与较旧的 Hewlett-Packard 1100 HPLC 连接的 ThermoElectron LCQ Duo）在我们的开放式实验室中运行，并且用户数量绝对饱和。另一台仪器是已有 13 年历史的 Fisons VG AutoSpec，主要通过液体二次离子 MS 进行标称和精确质量测量。新的 LC/MSD TOF 将满足对更多 HPLC/MS 容量以及更准确的质量测量能力的需求。我们预计，对于某些常规样品，我们的吞吐量将增加三到五倍，甚至可能更多。最重要的是，该过程可以自动化并设置为在仪器需求较低期间运行。新的 LC/MSD TOF 系统还允许我们仅通过一次操作即可测量所有感兴趣的离子（杂质、各种加合物和碎片），达到所需的精度。最后，其中许多测量将在开放访问环境中进行。