LC/MDS TOF SPECTROMETER FOR INSTRUMENT FACILITY AT CSU: CHEMISTRY

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：科罗拉多州立大学的化学系正在申请资金购买一台配备高效液相色谱（HPLC）系统的新飞行时间质谱仪（LC/MSD TOF）。该仪器将在该部的中央仪器设施内管理。目前，我们在两种大量使用的仪器上进行大部分的质谱分析工作，其中一种已经过了全盛时期。较新的仪器（ThermoElectron LCQ Duo与较旧的Hewlett-Packard 1100 HPLC接口）在我们的开放实验室中运行，用户绝对饱和。另一台仪器是有13年历史的Fisons VG AutoSpec，主要通过液体二次离子MS进行标称和准确的质量测量。新的LC/MSD TOF将满足对更多HPLC/MS容量以及更准确质量测量容量的需求。我们预计，对于某些常规样品，我们的吞吐量将增加三到五倍，甚至更多。最重要的是，该过程可以自动化，并设置为在较低的仪器需求期间运行。新的LC/MSD TOF系统还允许我们在一次操作中测量所有感兴趣的离子（杂质、各种加合物和碎片），达到所需的精度。最后，许多这些测量将在开放获取环境中进行。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Chemistry Department at Colorado State University is requesting funds to purchase a new time-of-flight mass spectrometer (LC/MSD TOF) equipped with a high performance liquid chromatography (HPLC) system. The instrument will be managed within the Department's Central Instrumentation Facility (GIF). At the present time, we do the greatest portion of our mass spectrometry work on two heavily used instruments, one of which is well past its prime. The newer instrument (a ThermoElectron LCQ Duo interfaced to an older Hewlett-Packard 1100 HPLC) operates in our open-access laboratory and is absolutely saturated with users. The other instrument is a thirteen year old Fisons VG AutoSpec set up to perform nominal and accurate mass measurements mostly by Liquid Secondary Ion MS. The new LC/MSD TOF will address the need for more HPLC/MS capacity as well as more accurate mass measurement capacity. We anticipate that for certain routine samples our throughput will increase by three to five times, maybe even more. Most importantly, the process can be automated and set to run during periods of lower instrument demand. The new LC/MSD TOF system will also allow us to measure all ions of interest (impurities, various adducts and fragments) to the required accuracy in only one operation. Finally, many of these measurements will be made in an open access environment.