LC/MDS TOF Spectrometer for Instrument Facility at CSU

用于科罗拉多州立大学仪器设施的 LC/MDS TOF 光谱仪

• 批准号: 7037975
• 负责人: Robert Michael Williams
• 金额: $ 26.05万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7037975
• 关键词: adduct; fluid; gait; high performance liquid chromatography; ions; mass spectrometry; measurement; motivation; quality of life; university

DESCRIPTION (provided by applicant): The Chemistry Department at Colorado State University is requesting funds to purchase a new time-of-flight mass spectrometer (LC/MSD TOF) equipped with a high performance liquid chromatography (HPLC) system. The instrument will be managed within the Department's Central Instrumentation Facility (GIF). At the present time, we do the greatest portion of our mass spectrometry work on two heavily used instruments, one of which is well past its prime. The newer instrument (a ThermoElectron LCQ Duo interfaced to an older Hewlett-Packard 1100 HPLC) operates in our open-access laboratory and is absolutely saturated with users. The other instrument is a thirteen year old Fisons VG AutoSpec set up to perform nominal and accurate mass measurements mostly by Liquid Secondary Ion MS. The new LC/MSD TOF will address the need for more HPLC/MS capacity as well as more accurate mass measurement capacity. We anticipate that for certain routine samples our throughput will increase by three to five times, maybe even more. Most importantly, the process can be automated and set to run during periods of lower instrument demand. The new LC/MSD TOF system will also allow us to measure all ions of interest (impurities, various adducts and fragments) to the required accuracy in only one operation. Finally, many of these measurements will be made in an open access environment.

描述（由申请人提供）：科罗拉多州立大学的化学系要求资金购买配备高性能液相色谱（HPLC）系统的新飞行时间质谱仪（LC/MSD TOF）。该工具将在该部门的中央仪器设施（GIF）内进行管理。目前，我们在两种大量使用的乐器上进行了质谱工作的最大部分，其中一种已经超过了它的素数。较新的仪器（Thermoelectron LCQ Duo连接到较旧的Hept-Packard 1100 HPLC）在我们的开放式实验室中运行，并且绝对充满了用户。另一种仪器是13岁的Fisons VG Autospec设置，用于进行名义和准确的质量测量，主要是液体次级离子MS。新的LC/MSD TOF将满足更多HPLC/MS容量以及更准确的质量测量能力的需求。我们预计，对于某些常规样本，我们的吞吐量将增加三到五倍，甚至更多。最重要的是，该过程可以自动化并设置为在较低的仪器需求期间运行。新的LC/MSD TOF系统还将使我们能够仅在一个操作中测量所有感兴趣的离子（杂质，各种加合物和片段）。最后，其中许多测量将在开放访问环境中进行。

项目成果

Mapping the valosin-containing protein (VCP) gene on human chromosome 9 and mouse chromosome 4, and a likely pseudogene on the mouse X chromosome.

绘制人类 9 号染色体和小鼠 4 号染色体上的缬洛辛蛋白 (VCP) 基因，以及小鼠 X 染色体上的一个可能的假基因。

• DOI: 10.1007/s003359900566
• 发表时间: 1997
• 期刊: Mammalian genome : official journal of the International Mammalian Genome Society
• 作者: Hoyle,J;Tan,KH;Fisher,EM
• 通讯作者: Fisher,EM