BIACORE T100

描述（由申请人提供）：我们正在申请共享仪器补助金，以购买BIACORE T100表面等离子体共振仪器，该仪器将用于测量体外分子相互作用。四个主要用户有项目研究：RNA运输中的分子相互作用，锚蛋白重复蛋白相互作用，分泌颗粒形成中的蛋白-蛋白相互作用和疱疹病毒复制中的分子相互作用。三个小用户有项目研究：细菌细胞分裂中的蛋白质-蛋白质相互作用，DNA错配修复中的分子相互作用和细菌菌毛形成中的分子相互作用。每个主要和次要的研究者都有用常规技术分析分子相互作用的经验。然而，他们的个别项目将受益于BIACORE T100仪器直接测量动力学结合常数的能力。BIACORE T100提供的定量数据将用作特定细胞生物学系统计算模型的输入参数。因此，BIACORE T100代表了细胞生物学过程计算建模的一种使能技术。 这项工作将促进人类健康，因为大多数（如果不是全部）细胞生物学过程是由可逆分子相互作用网络介导的。癌症、多发性硬化症和阿尔茨海默病等疾病是由特定相互作用网络的破坏引起的。为了理解一个特定的相互作用网络是如何运作的，有必要知道网络中的每个分子与其伙伴结合的速度以及它们解离的速度。我们要求的仪器BIACORE T100将使我们能够直接测量单个分子结合和解离的速度。这些定量信息将使我们能够了解分子相互作用网络如何介导特定的细胞生物学过程，以及这些网络如何在疾病中被破坏。

DESCRIPTION (provided by applicant): We are applying for a Shared Instrumentation Grant to purchase a BIACORE T100 surface plasmon resonance instrument, which will be used to measure molecular interactions in vitro. Four major users have projects studying: molecular intractions in RNA trafficking, ankyrin repeat protein interactions, protein-protein interactions in secretory granule formation and molecular interactions in herpes virus replication. Three minor users have projects studying: protein-protein interactions in bacterial cell division, molecular interactions in DMA mismatch repair and molecular interactions in bacterial pilus formation. Each of the major and minor investigators have experience analzying molecular interactions by conventional techniques. However, their individual projects will benefit by the capability of the BIACORE T100 instrument to directly measure kinetic binding constants. The quantitative data provided by the BIACORE T100 will be used as input parameters for computational models of specific cell biological systems. Thus, the BIACORE T100 represents an enabling technology for computational modeling of cell biological processes. This work will further human health as most, if not all, cell biological processes are mediated by networks of reversible molecular interactions. Diseases such as cancer, multiple sclerosis and Alzheimer's disease are caused by disruption of specific interaction networks. In order to understand how a particualr interaction network functions it is necessary to know how rapidly each molecule in the network binds to its partners and how rapidly they dissociate. The instrument we are requesting, the BIACORE T100, will enable us to directly measure how rapidly individual molecules associate and dissociate. This quantitative information will enable us to understand how molecular interaction networks mediate specific cell biological processes and how such networks are disrupted in disease