NUCLEOCYTOPLASMIC TRANSPORT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 蛋白质和核酸的核质转运对于正常细胞功能至关重要。活细胞中的荧光相关光谱（FCS）被用于直接测量输入蛋白β、输入蛋白α、NTF 2和Ran的组分的反应速率和移动的分数。FCS数据为每种核转运蛋白提供了必要的定量参数（浓度，扩散系数），这些蛋白被纳入核转运系统的更准确的虚拟细胞模型中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nucleocytoplasmic transport of proteins and nucleic acids is crucial for normal cell functioning. Fluorescence correlation spectroscopy (FCS) in live cells is being used to directly measure reaction rates and mobile fractions of components of importin beta, importin alpha, NTF2 and Ran. The FCS data provides essential quantitative parameters (concentrations, diffusion coefficients) for each of the nuclear transport proteins that are being incorporated into a more accurate Virtual Cell model for the nuclear transport system.