INTRACELLULAR RNA TRAFFICKING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 RNA运输是一个复杂的过程，通过这个过程，特定的RNA被定位到特定的亚细胞室。通过各种体内和体外技术，我们描绘了描述神经细胞中RNA运输系统的分子相互作用图。我们使用体外表面等离子体共振分析和荧光相关光谱(FCS)以及活细胞中的方法，测量了RNA转运中每个分子相互作用的定量参数。定量参数用于RNA运输的虚拟细胞模型

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking