INTRACELLULAR RNA TRAFFICKING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 RNA运输是一个复杂的过程，其中特定的RNA定位于特定的亚细胞区室。通过各种体内和体外技术，我们已经描绘了一个分子相互作用的地图，描述在神经细胞中的RNA运输系统。我们已经测量了定量参数的每个分子间的相互作用，在RNA贩运使用表面等离子体共振分析在体外和荧光相关光谱（FCS）和活细胞。在RNA运输的虚拟细胞模型中使用定量参数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking.