INTRACELLULAR RNA TRAFFICKING

细胞内 RNA 运输

• 批准号: 8362485
• 负责人: JOHN H CARSON
• 金额: $ 5.27万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362485
• 关键词: Binding; Cell model; Cells; Complex; Fluorescence; Funding; Grant; In Vitro; Life; Maps; Measures; Modeling; National Center for Research Resources; Neurons; Principal Investigator; Process; RNA; Research; Research Infrastructure; Resources; Source; Spectrum Analysis; Surface Plasmon Resonance; System; Techniques; United States National Institutes of Health; cost; in vivo; trafficking; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 RNA运输是一个复杂的过程，通过这个过程，特定的RNA被定位到特定的亚细胞室。通过各种体内和体外技术，我们描绘了描述神经细胞中RNA运输系统的分子相互作用图。我们使用体外表面等离子体共振分析和荧光相关光谱(FCS)以及活细胞中的方法，测量了RNA转运中每个分子相互作用的定量参数。定量参数用于RNA运输的虚拟细胞模型。