INTRACELLULAR RNA TRAFFICKING

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。RNA运输是一个复杂的过程，其中特定的RNA定位于特定的亚细胞区室。通过各种体内和体外技术，我们已经描绘了一个分子相互作用的地图，描述在神经细胞中的RNA运输系统。整个系统由5个子系统（核转运、颗粒组装、颗粒转运、pH调节和翻译调节）、40种分子种类和70种可逆分子相互作用组成。当单个分子物种参与多个相互作用时，条件运算符定义哪些反应是有利的。为了开发有意义的计算模型的RNA贩运，它是必要的，以测量浓度和扩散系数为每个分子种类，动力学常数为每个分子的相互作用和条件运营商的基板的多种相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. The overall system consists of 5 subsystems (nuclear transport, granule assembly, granule transport, pH regulation and translation regulation), 40 molecular species and 70 reversible molecular interactions. When individual molecular species participate in multiple interactions, conditional operators define which reaction(s) are favored. In order to develop meaningful computational models for RNA trafficking it is necessary to measure concentrations and diffusion coefficients for each molecular species, kinetic constants for each molecular interaction and conditional operators for substrates of multiple interactions.