INTRACELLULAR RNA TRAFFICKING

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。RNA运输是一个复杂的过程，通过这个过程，特定的RNA被定位到特定的亚细胞室。通过各种体内和体外技术，我们描绘了描述神经细胞中RNA运输系统的分子相互作用图。整个系统由5个子系统(核转运、颗粒组装、颗粒转运、pH调节和翻译调节)、40个分子物种和70个可逆的分子相互作用组成。当单个分子物种参与多个相互作用时，条件运算符定义了哪个反应(S)更受欢迎。为了建立有意义的RNA转运计算模型，有必要测量每个分子物种的浓度和扩散系数，每个分子相互作用的动力学常数和多个相互作用底物的条件算子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. The overall system consists of 5 subsystems (nuclear transport, granule assembly, granule transport, pH regulation and translation regulation), 40 molecular species and 70 reversible molecular interactions. When individual molecular species participate in multiple interactions, conditional operators define which reaction(s) are favored. In order to develop meaningful computational models for RNA trafficking it is necessary to measure concentrations and diffusion coefficients for each molecular species, kinetic constants for each molecular interaction and conditional operators for substrates of multiple interactions.