INTRACELLULAR RNA TRAFFICKING

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 RNA 运输是一个复杂的过程，特定的 RNA 被定位到特定的亚细胞区室。通过各种体内和体外技术，我们描绘了描述神经细胞中 RNA 运输系统的分子相互作用图。我们使用体外表面等离子体共振分析和荧光相关光谱 (FCS) 以及活细胞中的方法测量了 RNA 运输中每种分子相互作用的定量参数。定量参数用于 RNA 运输的虚拟细胞模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking.