Chlamydia trachomatis proteomics

沙眼衣原体蛋白质组学

基本信息

批准号：
7337131
负责人：
GUANGMING ZHONG
金额：
$ 33.95万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2009-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7337131
关键词：
Affinity Antibodies Antibody Formation Antigens Base Sequence Biological Biological Assay Cells Chimeric Proteins Chlamydia Chlamydia Infections Chlamydia trachomatis Clinical Clinical Data Cloning Communities Complement Cytosol Disease Frequencies Gene Expression Genes Genome Glutathione Glutathione S-Transferase Glycolipids Half-Life Human Immunity Immunodominant Antigens Immunoglobulin A Immunoglobulin G Individual Intervention Knowledge Lipopolysaccharides Location Maps Molecular Conformation Molecular Profiling Nucleic acid sequencing Open Reading Frames Pattern Play Post-Translational Protein Processing Prevention strategy Production Protein Array Protein Array Analysis Proteins Proteomics Reagent Recording of previous events Research Research Personnel Role Sampling Screening procedure Serum System Testing Vagina base genetic manipulation genome sequencing novel programs protein expression protein protein interaction response success

项目摘要

The available chlamydial genome sequences have made it possible to study chlamydia at the whole genome scale. For example, comparison of genome sequences have revealed functions of chlamydial genes with homologies to their counterparts in other species and provided information for chlamydial evolutionary history. Genome wide analyses of gene transcriptional profiles have correlated gene expression patterns with biological responses. However, these nucleic acid sequence-based analyses have limitations in fully understanding the functions of chlamydial proteins. Due to a lack of genetic manipulation for chlamydia, researchers are forced to develop function-driven genome wide screening assays in heterologous systems. While some of these assays have yielded useful information, many have met little success. To complement the above approaches for identifying functions of chlamydial proteins, we are proposing a functional proteomics approach, in which whole genome protein array assays will be developed for identifying protective and pathogenic determinants during human chlamydial infection and determining protein-protein interaction pairs. Furthermore, antibodies will be produced for characterizing endogenous chlamydial proteins for expression levels/patterns, half-life, post-translational modifications, interaction partners and intracellular localization. These proposed studies will provide essential information for understanding chlamydial pathogenic mechanisms and developing intervention and prevention strategies for controlling chlamydial infection-induced diseases.

可用的衣原体基因组序列使得在整个基因组量表上研究衣原体成为可能。例如，基因组序列的比较揭示了衣原体基因与其他物种中的同源物的功能，并为衣原体进化史提供了信息。基因组的基因转录谱分析具有与生物学反应相关的基因表达模式。但是，这些基于核酸序列的分析在完全理解衣原体蛋白的功能方面存在局限性。由于缺乏衣原体的基因操纵，研究人员被迫在异源系统中开发功能驱动的基因组广泛筛选测定法。尽管其中一些测定方法产生了有用的信息，但许多测定方法几乎没有成功。为了补充上述方法以识别衣原体蛋白的功能，我们是提出一种功能性蛋白质组学方法，其中将开发整个基因组蛋白阵列分析，以鉴定人衣原体感染期间的保护性和致病性决定因素并确定蛋白质 - 蛋白质相互作用对。此外，将生产用于表征内源性衣原体蛋白的抗体，用于表达水平/模式，半衰期，翻译后修饰，相互作用伴侣和细胞内定位。这些拟议的研究将为理解衣原体的致病机制以及制定控制衣原体感染引起的疾病的干预和预防策略提供必不可少的信息。