Chlamydial Manipulation of Host Apoptosis

衣原体操纵宿主细胞凋亡

• 批准号: 7339909
• 负责人: GUANGMING ZHONG
• 金额: $ 31.29万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339909
• 关键词: Affinity; Animals; Antibodies; Apoptosis; Atherosclerosis; Bacterial Sexually Transmitted Diseases; Binding; Biological Assay; Blood Vessels; Categories; Cell Survival; Cells; Centers for Disease Control and Prevention (U.S.); Cerebrum; Cervix carcinoma; Chlamydia; Chlamydia Infections; Chlamydophila pneumoniae; Chlamydophila psittaci; Clinical; Complement; Complex; Condition; Cultured Cells; Cytosol; Developed Countries; Developing Countries; Endopeptidases; Ensure; Eukaryotic Cell; Expression Library; Feces; Figs - dietary; Future; Genes; Genitourinary system; Genome; Genomic Library; Goals; Growth; Half-Life; Health; Host Defense; Human; Immunity; Induction of Apoptosis; Infection; Life; Life Cycle Stages; Link; Location; Mammalian Cell; Maps; Organism; Pathology; Peptide Hydrolases; Peptides; Phage Display; Play; Pneumonia; Proteins; Random Peptide Libraries; Reagent; Research Personnel; Resistance; Respiratory Tract Infections; Role; Scheme; Screening procedure; Sequence Analysis; Stimulus; Structure; Study Section; Technology; Testing; Time; Vacuole; Virus; Work; aerosolized; base; biodefense; cofactor; design; experience; improved; inhibitor/antagonist; microbial; pathogen; prevent; programs; success; trafficking

Mechanisms of Chlamydial Manipulation of Host Cell Apoptosis Chlamydial infection in humans imposes a major health problem in both developing and developed nations. Urogenital tract infection with C. trachomatis species is a leading cause of sexually transmitted bacterial diseases and is also linked to certain type of cervical carcinoma while respiratory infection with C. pneumoniae species is associated with atherosclerosis, a major vascular condition for cardio-cerebral fatality. Although the species C. psittaci is primarily an animal pathogen, humans are also susceptible to C. psittaci infection, developing life-threatening pneumonia. Since humans can acquire infection via aerosolized animal feces that are contaminated with C. psittaci organisms, CDC has once listed C. psittaci as a category B agent for biodefense. These chlamydia-induced or -associated pathologies are largely due to Chlamydial ability to either productively replicate or to achieve a long-term persistence within a cytoplasmic vacuole of eukaryotic cells, which are aided by the Chlamydial unique intracellular biphasic life cycle and the Chlamydial ability to evade host defense. The current proposal is designed to understand how chlamydia evades a very important host defense effector mechanism¿apoptosis. We have previously demonstrated that chlamydia possesses a potent antiapoptotic activity, which may contribute to the Chlamydial ability to survive in the infected hosts for long periods of time. By identifying the molecule(s) responsible for the Chlamydial antiapoptotic activity and understanding how the antiapoptotic molecules work as proposed in the current project, we may be able to develop reagents/approaches for blocking the Chlamydial antiapoptotic activity and preventing chlamydia-induced pathologies.

宿主细胞凋亡的衣原体操纵机制 人类的衣原体感染不可能在发展中和 发达国家。泌尿生殖道被沙眼梭状芽孢杆菌感染是 性传播细菌疾病，也与某些类型的宫颈癌有关 肺炎梭状芽胞杆菌的呼吸道感染与动脉粥样硬化有关，这是主要的 心脏脑死亡的血管状况。尽管C. psittaci物种是主要动物 病原体，人类也容易受到C. psittaci感染的影响，发展生命 肺炎。由于人类可以通过被污染的动物粪便获得感染 使用C. psittaci生物，CDC曾经将C. psittaci列为生物粘剂B类代理。 这些衣原体引起或相关的病理主要是由于衣原体的能力 有效地复制或实现长期持久性的细胞质真空吸尘器 真核细胞，由衣原体独特的细胞内双相生命周期和 衣原体能够逃避东道国防御。当前的建议旨在了解如何 衣原体逃避了一种非常重要的宿主防御效应子机制。细胞凋亡。我们有 以前证明衣原体具有有效的抗凋亡活性，这可能 有助于长期在感染宿主中生存的衣原体能力。经过 识别负责衣原体抗凋亡活性的分子和理解 抗凋亡分子如何按照当前项目提出的工作，我们可能能够 开发用于阻断衣原体抗凋亡活性并防止的试剂/方法 衣原体引起的病理。