Chlamydia trachomatis proteomics

沙眼衣原体蛋白质组学

基本信息

批准号：
8423042
负责人：
GUANGMING ZHONG
金额：
$ 34.9万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8423042
关键词：
Animal Model Antibodies Antibody Formation Antigens B-Lymphocytes Bacteriophages Biological Biological Models Cell Culture Techniques Cells Chimeric Proteins Chlamydia Chlamydia muridarum Chlamydia trachomatis Communities Complement Control Groups Detection Development Diagnosis Diagnostic Diagnostic Reagent Expression Library Foundations Funding Gel Genital system Genitourinary system Genome Grant Human Immune response Immunity Immunodominant Antigens Individual Infection Infertility Inflammatory Knowledge Lead Maps Molecular Conformation Mus Open Reading Frames Organism Pathogenesis Pathology Pathway interactions Patients Peripheral Blood Mononuclear Cell Pilot Projects Plasmids Progress Reports Protein Array Proteins Proteome Proteomics Reagent Recruitment Activity Research Resolution Sampling Sampling Studies Specificity Subunit Vaccines T cell response T-Lymphocyte Testing Vaccines Woman major outer membrane protein novel prognostic public health relevance research study response tool

项目摘要

DESCRIPTION (provided by applicant): C. trachomatis proteomics: Whole genome scale mapping of C. trachomatis protective & pathogenic determinants. Chlamydia trachomatis urogenital tract infection causes inflammatory pathologies in the upper genital tract, which can lead to complications including tubal factor infertility. Understanding the pathogenic mechanisms and developing subunit vaccines have been the major focuses in modern chlamydial research. However, knowledge on chlamydial pathogenesis remains limited and there is no effective vaccine. This may be mainly due to limitations in the approaches previously used for analyzing chlamydial antigens. Although these previous approaches have provided useful information, for example, revealing the association of anti-MOMP antibody responses with protective immunity and anti-HSP60 antibody responses with pathogenesis, the results are limited in scope. To make significant advancements in understanding chlamydial pathogenic mechanisms and developing effective subunit vaccines, Dr. Zhong's lab expressed all C. trachomatis open reading frames (ORFs) as soluble fusion proteins and developed a high quality whole genome scale proteome array in the past 5 years. This array has allowed the detection of both linear and conformation-dependent human antibodies. Using this array, Dr. Zhong's lab has mapped the B cell ANTIGENome and identified novel immunodominant antigens of C. trachomatis. In the current grant, Dr. Zhong will use the whole genome scale proteome array to profile the antigen specificities of both antibody and T cell responses in C. trachomatis infected-women and to identify antigens that are associated with pathology or protective immunity. The biological relevance of the identified antigens will be characterized in both cell culture and animal model systems. Both the knowledge gained and reagents accumulated from the proposed studies will be made available to the research community. The information obtained will lay the foundation for both understanding chlamydial pathogenic mechanisms and developing diagnostic reagents and subunit vaccines.

描述（由申请人提供）：沙眼蛋白酶蛋白质组学：沙眼梭状芽孢杆菌保护和致病性决定因素的全基因组尺度映射。沙眼衣原体泌尿生殖道感染会导致上生殖道的炎症性病理，这可能导致并发症，包括管子因子不育症。了解致病机制和开发亚基疫苗一直是现代衣原体研究的主要重点。但是，关于衣原体发病机理的知识仍然有限，并且没有有效的疫苗。这可能主要是由于先前用于分析衣原体抗原的方法的局限性。尽管这些先前的方法提供了有用的信息，但例如，揭示了抗MOMP抗体反应与保护性免疫和抗HSP60抗体反应与发病机理的关联，但结果的范围受到限制。为了在理解衣原体的致病机制和开发有效的亚基疫苗方面取得重大进步，钟博士的实验室表达了所有的沙眼开放式读取框（ORF）作为可溶性融合蛋白，并在过去5年中开发了高质量的整个基因组量表蛋白质组阵列。该阵列允许检测线性和构象依赖性人类抗体。使用此阵列，中东博士的实验室绘制了B细胞抗原组，并鉴定出新型的沙眼曲霉的免疫主导抗原。在当前的赠款中，钟博士将使用整个基因组量表蛋白质组阵列来介绍抗体和沙眼感染的抗体反应的抗原特异性，并鉴定与病理或保护性免疫相关的抗原。在细胞培养和动物模型系统中，鉴定出的抗原的生物学相关性将被表征。研究界将获得所提出的研究的知识和从拟议的研究中积累的试剂。获得的信息将为了解衣原体的致病机制和开发诊断试剂和亚基疫苗奠定基础。