Chlamydial Manipulation of Host Apoptosis

衣原体操纵宿主细胞凋亡

• 批准号: 7760842
• 负责人: GUANGMING ZHONG
• 金额: $ 30.98万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2012-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7760842
• 关键词: Affinity; Animals; Antibodies; Apoptosis; Atherosclerosis; Bacterial Sexually Transmitted Diseases; Binding; Biological Assay; Blood Vessels; Categories; Cell Culture Techniques; Cell Survival; Cells; Centers for Disease Control and Prevention (U.S.); Cerebrum; Cervix carcinoma; Chlamydia; Chlamydia Infections; Chlamydophila pneumoniae; Chlamydophila psittaci; Clinical; Complement; Complex; Cytosol; Developed Countries; Ensure; Eukaryotic Cell; Expression Library; Feces; Figs - dietary; Future; Genes; Genitourinary system; Genome; Genomic Library; Goals; Growth; Half-Life; Health; Host Defense; Human; Induction of Apoptosis; Infection; Life; Life Cycle Stages; Link; Location; Mammalian Cell; Maps; Organism; Pathology; Peptide Hydrolases; Peptides; Phage Display; Play; Pneumonia; Proteins; Random Peptide Libraries; Reagent; Research Personnel; Resistance; Respiratory Tract Infections; Role; Scheme; Screening procedure; Sequence Analysis; Stimulus; Structure; Study Section; Technology; Testing; Time; Vacuole; Virus; Work; adaptive immunity; aerosolized; base; biodefense; cofactor; design; experience; genome-wide; improved; inhibitor/antagonist; microbial; pathogen; prevent; programs; success; trafficking

Mechanisms of Chlamydial Manipulation of Host Cell Apoptosis Chlamydial infection in humans imposes a major health problem in both developing and developed nations. Urogenital tract infection with C. trachomatis species is a leading cause of sexually transmitted bacterial diseases and is also linked to certain type of cervical carcinoma while respiratory infection with C. pneumoniae species is associated with atherosclerosis, a major vascular condition for cardio-cerebral fatality. Although the species C. psittaci is primarily an animal pathogen, humans are also susceptible to C. psittaci infection, developing life-threatening pneumonia. Since humans can acquire infection via aerosolized animal feces that are contaminated with C. psittaci organisms, CDC has once listed C. psittaci as a category B agent for biodefense. These chlamydia-induced or -associated pathologies are largely due to Chlamydial ability to either productively replicate or to achieve a long-term persistence within a cytoplasmic vacuole of eukaryotic cells, which are aided by the Chlamydial unique intracellular biphasic life cycle and the Chlamydial ability to evade host defense. The current proposal is designed to understand how chlamydia evades a very important host defense effector mechanism¿apoptosis. We have previously demonstrated that chlamydia possesses a potent antiapoptotic activity, which may contribute to the Chlamydial ability to survive in the infected hosts for long periods of time. By identifying the molecule(s) responsible for the Chlamydial antiapoptotic activity and understanding how the antiapoptotic molecules work as proposed in the current project, we may be able to develop reagents/approaches for blocking the Chlamydial antiapoptotic activity and preventing chlamydia-induced pathologies.

衣原体操纵宿主细胞凋亡的机制 人类衣原体感染给发展中国家和发展中国家带来了重大健康问题。 发达国家。沙眼衣原体泌尿生殖道感染是导致以下疾病的主要原因 性传播细菌性疾病，也与某些类型的宫颈癌有关 肺炎衣原体的呼吸道感染与动脉粥样硬化有关，动脉粥样硬化是一种主要的疾病 心脑死亡的血管状况。尽管鹦鹉热鹦鹉物种主要是一种动物 病原体，人类也容易受到鹦鹉热衣原体感染，从而危及生命 肺炎。由于人类可以通过被污染的动物粪便雾化而受到感染 对于鹦鹉热衣原体，CDC 曾将鹦鹉热衣原体列为 B 类生物防御剂。 这些衣原体引起的或相关的病理很大程度上是由于衣原体能够 有效地复制或在细胞质液泡内实现长期持续存在 真核细胞，受到衣原体独特的细胞内双相生命周期和 衣原体逃避宿主防御的能力。当前的提案旨在了解如何 衣原体逃避一种非常重要的宿主防御效应机制——细胞凋亡。我们有 先前证明衣原体具有有效的抗细胞凋亡活性，这可能 有助于衣原体在受感染宿主体内长期生存的能力。经过 识别负责衣原体抗凋亡活性的分子并了解 抗凋亡分子如何按照当前项目中提出的方式发挥作用，我们也许能够 开发阻断衣原体抗凋亡活性并预防的试剂/方法 衣原体引起的病理。