GNRH II STIMULATION AND SEXUAL DYSFUNCTION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。为了提高我们对狨猴神经内分泌机制的理解，阐明GnRH肽作为雌性反应的非雌激素刺激物的功效，并有助于制定更合适的女性性功能障碍临床干预措施。性功能障碍影响了大约43%的女性。目前还没有批准的药物治疗这种疾病。对雌激素和孕激素激素治疗不安全的进一步关注促使人们寻求非甾体药物治疗。神经肽促性腺激素释放激素II在一系列非灵长类动物中刺激性行为，但其在人类和非人类灵长类动物中的作用尚不清楚。我们开发了一个雌性普通狨猴模型系统，以确定对GnRH II的行为反应。与对照组相比，雌性对其伴侣表现出的感受性（性引诱）行为（甩舌头、感受性的凝视和静止的姿势）的频率增加了近四倍。GnRH II增加了性接受度（雌性使雄性发生性行为），通过将雄性的插入频率增加一倍，而不影响骑乘或尝试骑乘的频率。GnRH II对性行为的刺激不依赖雌二醇。这些发现表明GnRH II在雌性灵长类动物性行为的调节中起作用。供资在本报告所述期间之前结束，本进度报告是关于结果发现和出版的。本研究使用了WNPRC化验服务和动物服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To improve our understanding of neuroendocrine mechanisms in marmosets to clarify the efficacy of GnRH peptides as non-estrogenic stimulators of female response and aid in developing more appropriate clinical intervention for sexual dysfunction in women. Sexual dysfunction affects an estimated 43% of women. Currently there are no approved drug therapies for this disorder. Further concerns that hormonal treatment with estrogens and progestogens are not safe motivates the search for non-steroidal pharmoacotherapy. Neuropeptide gonadotropin releasing hormone II stimulates sexual behavior in a range of non-primates, but its role in humans and nonhuman primates is unknown. We developed a model system with female common marmosets to determine the behavioral responses to GnRH II. GnRH II increases nearly four-fold the frequency of proceptive (sexual solicitation) behaviors (tongue flicking, proceptive stares and frozen postures) exhibited by females towards their pair-mates compared to controls. GnRH II increases sexual receptivity (females enabling male sexual behavior) by doubling the frequency of intromission by males compared to vehicle without affecting mount or mount attempt frequencies. GnRH II stimulation of sexual behavior is not estradiol dependent. These findings indicate a role for GnRH II in the regulation of female primate sexual behavior. The funding ended before this reporting period and this progress report is on the resulting findings and publication. This research used WNPRC Assay Services and Animal Services.