ENGINEERED GLUCOSE METABOLISM IN INSULIN SECRETING CELLS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。纽加德实验室使用跨学科方法研究葡萄糖刺激的胰岛素分泌所涉及的生化和分子机制，包括开发新的细胞模型、基因发现和基因工程。该项目目前的研究重点是改变丙酮酸循环(通过13C核磁共振测量)在葡萄糖刺激的胰岛素分泌(GSIS)损害中的潜在作用，例如糖尿病。《资源》杂志之前的研究使用13C核磁共振显示，在高游离脂肪酸(1 mM油酸：棕榈酸混合物)中进行长期(48h)培养的强健的葡萄糖反应细胞系显示丙酮酸循环和GSIS减少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Newgard laboratory uses interdisciplinary approaches for the study of the biochemical and molecular mechanisms involved in glucose-stimulated insulin secretion, including development of novel cellular models, gene discovery and genetic engineering. Current studies in this project focus on the potential role of altered pyruvate cycling (as measured by 13C NMR) in impairment of glucose-stimulated insulin secretion (GSIS) such as found in diabetes. Previous studies in the Resource used 13C NMR to show that robustly glucose responsive cell lines subjected to chronic (48 h) culture in elevated free fatty acids (1 mM oleate:palmitate mixture) displayed decreased pyruvate cycling and GSIS.