SITE AND LOOP DIRECTED MUTAGENESIS STUDIES OF 2FE-2S FERREDOXINS

2FE-2S 铁氧还蛋白的位点和环定向诱变研究

• 批准号: 7420592
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-20 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420592
• 关键词: hydrogen ions; mutant; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 1D proton spectra will be acquired for the mutant proteins prepared, specifically, the loop swap mutant given the fact that the reduced form is not EPR active. The proton spectrum will help to assess whether there has been a significant change in the local electronic structure of the residues surrounding the cluster.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。将获得制备的突变体蛋白质的1D质子光谱，特别是考虑到还原形式不具有EPR活性的事实的环交换突变体。质子光谱将有助于评估团簇周围残基的局部电子结构是否发生了重大变化。