CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

细胞表面糖脂 SSEA-3 和 SSEA-4

• 批准号: 7359038
• 负责人: ALFRED Harrison MERRILL
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359038
• 关键词: CELL; SURFACE; GLYCOSPHINGOLIPIDS; SSEA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ESI-MS/MS was used to measure sphingolipids and glycosphingolipids of human ES cells that have been treated with reagents that block the generation of SSEA-3 and -4. Depletion of these species and their precursors was confirmed using immunostaining and flow cytometry. The cells otherwise appeared normal based on transcriptional analysis, immunostaining, differentiation in vitro and in teratomas; therefore, it appears that SSEA-3 and -4 do not play critical functional roles in maintaining the pluripotency of human ES cells.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。ESI-MS/MS用于测量已经用阻断SSEA-3和-4产生的试剂处理的人ES细胞的鞘脂和鞘糖脂。使用免疫染色和流式细胞术证实这些物质及其前体的耗尽。基于转录分析、免疫染色、体外和畸胎瘤中的分化，细胞在其他方面表现正常;因此，似乎SSEA-3和SSEA-4在维持人ES细胞的多能性方面不起关键的功能作用。