CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。ESI-MS/MS用于测定经阻断SSEA-3和-4生成的试剂处理后的人ES细胞的鞘糖脂和神经鞘糖脂。用免疫染色和流式细胞术证实了这些物种及其前体的耗竭。根据转录分析、免疫染色、体外分化和畸胎瘤中的其他结果，这些细胞看起来正常；因此，SSEA-3和-4似乎在维持人ES细胞的多能性方面不起关键作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ESI-MS/MS was used to measure sphingolipids and glycosphingolipids of human ES cells that have been treated with reagents that block the generation of SSEA-3 and -4. Depletion of these species and their precursors was confirmed using immunostaining and flow cytometry. The cells otherwise appeared normal based on transcriptional analysis, immunostaining, differentiation in vitro and in teratomas; therefore, it appears that SSEA-3 and -4 do not play critical functional roles in maintaining the pluripotency of human ES cells.