EFFECTS OF MEALS OF VARYING FAT&FIBER CONTENT ON POSTPRANDIAL TESTOSTERONE CONC

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。饮食和生活方式的改变可以改善多囊卵巢综合征（PCOS）的症状，包括高雄激素血症和月经紊乱。然而，没有研究评估PCOS女性对不同饮食组成的餐后反应。主要目的是确定不同脂肪和纤维含量的膳食是否会影响PCOS女性的血浆睾酮浓度。第二个目的是观察不同脂肪和纤维含量的膳食是否影响循环葡萄糖、胰岛素和促性腺激素（LH和FSH）浓度。我们将招募19-40岁的多囊卵巢综合征（慢性少排卵/无排卵和高雄激素血症）女性患者，这些患者未服用任何混淆药物（激素避孕药、胰岛素增敏剂、抗高血压药、抗精神病药）或目前妊娠。所有受试者将填写知情同意书和病史，并接受体格检查、双能X线吸收仪（DXA）和经阴道超声检查。受试者将在15分钟内食用试验餐。这顿饭将包括：（1）鸡蛋和奶酪羊角面包和全脂牛奶或（2）谷物，低脂牛奶和苹果或香蕉，酸奶。每餐后，将每10分钟采集3 mL血样，持续6小时用于FSH和LH测量，并在30分钟和每小时采集20 mL血样用于葡萄糖、胰岛素、睾酮和SHBG测量。将使用MiniMed持续葡萄糖监测系统持续监测葡萄糖水平。参与者将参加这两种情况，测试餐至少间隔七天。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Changes in diet and lifestyle can improve symptoms of polycystic ovary syndrome (PCOS), including hyperandrogenemia and menstrual disturbances. However, no studies have evaluated the postprandial response to meals of varying diet composition in women with PCOS. The primary objective is to determine if meals of different fat and fiber content will affect plasma testosterone concentrations in women with PCOS. The second objective is to see if meals of different fat and fiber content affect circulating glucose, insulin and gonadotropin (LH and FSH) concentrations. We will recruit women age 19-40 with polycystic ovary syndrome (chronic oligo/anovulation and hyperandrogenemia) who are not taking any confounding medications (hormonal contraceptives, insulin sensitizing agents, antihypertensives, antipsychotics) or are currently pregnant. All subjects will complete an informed consent and medical history and undergo a physical exam, dual energy x-ray absorptiometery (DXA) and transvaginal ultrasound. Subjects will be served the test meal that is to be consumed within 15 minutes. The meal will either consist of: (1) egg and cheese croissant and whole milk or (2) cereal, low-fat milk and an apple or banana, yogurt. After each meal, 3-mL blood samples will be taken every 10 minutes for 6 hours for FSH and LH measures and at 30 minutes and every hour, 20 mL will be taken for glucose, insulin, testosterone and SHBG. Glucose levels will be monitored continuously using the MiniMed Continuous Glucose Monitoring System. Participants will participate in both conditions with test meals being separated by at least seven days.