IMPACT OF HYDRATION STATUS ON INTERPRETATION OF TESTS TO ASSESS ADRENAL FUNCTION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。本研究的主要目的是确定水合作用和血管内容量变化对健康志愿者肾上腺功能参数的影响。这些参数包括血清总皮质醇和游离皮质醇水平、唾液皮质醇浓度以及血清皮质类固醇结合球蛋白（CBG）和白蛋白水平。该研究将检验以下假设：静脉注射（IV）液体扩大血管内容量会导致包括白蛋白和CBG在内的血浆蛋白血液稀释，随后导致测得的血清皮质醇水平下降，但不会导致血清游离皮质醇或唾液皮质醇浓度下降。其目标是：1）检查健康受试者快速静脉输液对血清皮质醇、游离皮质醇、CGB、白蛋白和唾液皮质醇浓度测量值的影响，2）将血清皮质醇、CGB和白蛋白的下降与其他血管内容量参数（如尿素、肌酐、尿酸和血红蛋白）的下降相关联。这项研究将涉及10名年龄在21至50岁之间的健康男性和女性。受试者将在GCRC中插入导管，并将接受每小时500 ml乳酸林格氏液IV给药，持续2小时。将在3小时内采集生命体征以及血液和唾液样本。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary objective of this investigator-initiated, single-site study is to determine the influence of hydration and changes in intravascular volume on parameters used to define adrenal function in healthy volunteers. The parameters include serum total and free cortisol levels, salivary cortisol concentration as well as serum corticosteroid-binding globulin (CBG) and albumin levels. The study will test the hypothesis that expansion of the intravascular volume with intravenous (IV) fluids results in hemodilution of plasma proteins including albumin and CBG and subsequently leads to a decrease in measured serum cortisol levels but not in serum free cortisol or salivary cortisol concentrations. The aims are: 1) to examine the influence of rapid IV fluid administration to healthy subjects on the concentration of serum cortisol, free cortisol, CGB, albumin, and salivary cortisol measurements, and 2) to correlate the decrement in serum cortisol, CGB, and albumin with those noted in other parameters of intravascular volume such as urea, creatinine, uric acid, and hemoglobin. The study will involve 10 healthy men and women age 21 to 50. Subjects will be catheterized in the GCRC and will be given an IV solution of 500 ml Lactated Ringers per hour for two hours. Vital signs as well as blood and saliva samples will be obtained over 3 hours.