IMPACT OF HYDRATION STATUS ON INTERPRETATION OF TESTS TO ASSESS ADRENAL FUNCTION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项由研究者发起的单点研究的主要目的是确定水合作用和血管内容量的变化对用于确定健康志愿者肾上腺功能的参数的影响。这些参数包括血清总皮质醇和游离皮质醇水平、唾液皮质醇浓度以及血清皮质类固醇结合球蛋白(CBG)和白蛋白水平。这项研究将检验这样一种假设，即静脉(IV)液体扩大血管内容量会导致包括白蛋白和CBG在内的血浆蛋白的血液稀释，从而导致测量的血清皮质醇水平下降，但不会降低血清游离皮质醇或唾液皮质醇浓度。其目的是：1)检查健康受试者快速静脉输液对血清皮质醇、游离皮质醇、CGB、白蛋白和唾液皮质醇浓度的影响，以及2)将血清皮质醇、CGB和白蛋白的降低与其他血管内容量参数如尿素、肌酐、尿酸和血红蛋白的相关性。这项研究将涉及10名年龄在21岁至50岁之间的健康男性和女性。受试者将在GCRC中进行导尿，并给予每小时500毫升乳酸林格液静脉滴注，持续两小时。生命体征以及血液和唾液样本将在3个小时内采集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary objective of this investigator-initiated, single-site study is to determine the influence of hydration and changes in intravascular volume on parameters used to define adrenal function in healthy volunteers. The parameters include serum total and free cortisol levels, salivary cortisol concentration as well as serum corticosteroid-binding globulin (CBG) and albumin levels. The study will test the hypothesis that expansion of the intravascular volume with intravenous (IV) fluids results in hemodilution of plasma proteins including albumin and CBG and subsequently leads to a decrease in measured serum cortisol levels but not in serum free cortisol or salivary cortisol concentrations. The aims are: 1) to examine the influence of rapid IV fluid administration to healthy subjects on the concentration of serum cortisol, free cortisol, CGB, albumin, and salivary cortisol measurements, and 2) to correlate the decrement in serum cortisol, CGB, and albumin with those noted in other parameters of intravascular volume such as urea, creatinine, uric acid, and hemoglobin. The study will involve 10 healthy men and women age 21 to 50. Subjects will be catheterized in the GCRC and will be given an IV solution of 500 ml Lactated Ringers per hour for two hours. Vital signs as well as blood and saliva samples will be obtained over 3 hours.