COBRE KS: FERMENTATION & SCREENING CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。鉴定在微生物复制或发病机制中具有重要作用的蛋白质的抑制剂的关键之一是具有它们的晶体结构。核心C的建立是为了提供生产大量蛋白质的发酵能力，用于晶体学研究。几种蛋白质已经过度生产，核心C正在与核心B密切合作，开始几种蛋白质的结晶研究，包括细菌细胞分裂的抑制剂。聘请了一位在蛋白质纯化方面有经验的科学家。向所有调查人员提供援助，包括进行净化、提供咨询意见和提供随时可用的设备。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the keys to identify inhibitors of proteins that have an essential role in microbial replication or pathogenesis is to have their crystal structures. Core C has been set up to provide the fermentation capacity for the production of large amounts of proteins for crystallographic studies. Several proteins have been overproduced and Core C is working closely with Core B to begin crystallization studies of several proteins, including inhibitors of bacterial cell division. A scientist has been hired who has experience in protein purification. Assistance is offered to all investigators, including doing the purification, offering advice and making the equipment readily available.