CELL CYCLE REGULATION OF GENE EXPRESSION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该项目将分析细胞周期调节蛋白对特定抑制剂的反应变化。我们将用二甲双胍等抑制剂处理正常细胞和乳腺癌细胞系，然后观察其对细胞周期蛋白D启动子和p27表达的影响。这项工作将包括分离质粒DNA、转染培养细胞和各种生化分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will involve analysis of changes in cell cycle regulatory proteins in response to specific inhibitors. We will treat normal cells and breast cancer cell lines with inhibitors such as metformin and then observe the effects on the cyclin D promoter and p27 expression. The work will involve isolating plasmid DNAs, transfection of cultured cells and various biochemical assays.