COBRE: LSC HSC: HISTOLOGY CORE

COBRE：LSC HSC：组织学核心

• 批准号: 7382069
• 负责人: JACK P STRONG
• 金额: $ 26.78万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382069
• 关键词: COBRE; LSC; HSC; HISTOLOGY; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Director ¿ Jack Strong, M.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, via the efforts of Drs. Strong and Huber, provides all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。组织学核心主任杰克·斯特朗，医学博士组织学核心是在杰克·斯特朗的指导下，他是一位在组织学和心血管病理学的各个方面都有丰富经验的研究者。这个组织学核心负责组织处理和染色，动物模型的病理分析以及细胞培养，以及组织和细胞样本的原位杂交和免疫细胞化学。这个核心被所有初级研究人员用于细胞和组织分析。组织切片由固定液灌注的动物或快速冷冻的器官产生。我们还通过访问LSUHSC形态学核心设施中的额外资源扩展了这一资源。该核心中的组织处理设备包括Hypercenter XP处理器；组织中心石蜡包埋站；Finesse石蜡切片机；一台Leica cm3050s低温恒温器，配有用于全鼠切片的碳化钨刀和EMS振荡组织切片机。组织学核心提供了以下服务：1。心脏、肾脏和血管组织加工；组织的苏木精、伊红和亚甲基蓝染色。2. 免疫组化染色大鼠对照和受损动脉组织（平滑肌肌动蛋白和Erk-1）。3. 利用平滑肌肌动蛋白4评价损伤与对照大鼠动脉组织及其对血管内膜平滑肌细胞的影响。基于免疫组化的损伤与对照动脉组织中t细胞和巨噬细胞的评估TUNEL染色分析心肌细胞损伤和死亡。核心实验室，通过博士的努力。Strong and Huber提供了所有适当的技术和经过专门培训的人员，从各种固定的人类和小鼠动脉标本和不同基质上的细胞培养物中，在显微镜和分子水平上评估这些标本。这一核心设施的使用将在未来一年继续增长。