COBRE: LSC HSC: HISTOLOGY CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Director ¿ Jack Strong, M.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, via the efforts of Drs. Strong and Huber, provides all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.