COBRE: LSC HSC: HISTOLOGY CORE

COBRE：LSC HSC：组织学核心

• 批准号: 7610599
• 负责人: JACK P STRONG
• 金额: $ 9.06万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610599
• 关键词: Animal Model; Animals; Cardiac; Cardiovascular Pathology; Cells; Cessation of life; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cultured Cells; Doctor of Medicine; Doctor of Philosophy; Equipment; Evaluation; Fixatives; Freezing; Funding; Grant; Hematoxylin and Eosin Staining Method; Histology; Human; Human Resources; In Situ Hybridization; In Situ Nick-End Labeling; Injury; Institution; Methylene blue; Microscopic; Microtome - medical device; Molecular; Morphology; Mus; Myocardium; Organ; Paraffin; Paraffin Embedding; Pathology; Postdoctoral Fellow; Rattus; Research; Research Personnel; Resources; Sampling; Services; Smooth Muscle Actin Staining Method; Smooth Muscle Myocytes; Source; Specimen; Staining method; Stains; T-Lymphocyte; Technology; Tissue Stains; Tissues; Training; Tunica Intima; United States National Institutes of Health; base; cell injury; cryostat; experience; finesse; immunocytochemistry; kidney vascular structure; macrophage; tissue processing; tungsten carbide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Jack Strong, Ph.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, through the efforts of Drs. Strong, Carmen Espinoza, and Dana Troxclair as well as Research Associate Penelope Strenge provide all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 组织学核心 杰克·斯特朗博士。 组织学核心由杰克·P·斯特朗医学博士领导，他是一名在组织学和心血管病理学的各个方面都有丰富经验的调查员。该组织学核心负责组织处理和染色、动物模型和细胞培养的病理分析，以及组织和细胞样本的原位杂交和免疫细胞化学。这个核心被所有初级研究人员用于细胞和组织分析。组织切片要么来自灌流固定剂的动物，要么来自快速冷冻的器官。我们还通过在LSUHSC形态核心设施中获得更多资源来扩大这一资源。该核心中的组织处理设备包括Hypercenter XP处理器；Histocentre石蜡包埋站；FINESSE石蜡切片机；配备了用于全鼠切片的碳化钨刀的徕卡CM3050 S低温恒温器和EMS振荡组织切片机。 组织学核心提供了以下服务： 1.心脏、肾脏和血管组织处理；组织苏木精-伊红和亚甲蓝染色。 2.大鼠对照组和损伤动脉组织(平滑肌肌动蛋白和ERK-1)的免疫组织化学染色。 3.损伤对照大鼠动脉组织的评价及其对血管内膜平滑肌细胞的影响 4.损伤对照动脉组织中T细胞和巨噬细胞的免疫组织化学检测 5.原位末端标记法检测心肌细胞损伤和死亡情况。 通过Strong博士、Carmen Espinoza博士和Dana Troxclair博士以及研究助理Penelope Strengg的努力，核心实验室提供了所有适当的技术和受过专门培训的人员，从不同基质上的各种固定的人和鼠动脉标本和细胞培养中对这些标本进行显微和分子水平的评估。这一核心设施的使用在未来一年将继续增长。