OH RADICAL OXIDATION DURING ESI

ESI 期间的 OH 自由基氧化

• 批准号: 7355151
• 负责人: MICHAEL L GROSS
• 金额: $ 0.71万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355151
• 关键词: OH; RADICAL; OXIDATION; DURING; ESI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in developing mass spectral methods to follow and uncover chemistry of biological materials. We wish to do this "in-situ;" that is, in the process of conducting MALDI or ESI. Free radical damage is one area where considerable inferential evidence points to the consequences of free radicals in damaging DNA and proteins. We believe that direct evidence of reactivity of biological material with free radicals could be gained by conducting the damaging chemical reactions in the electrospray source. To this end, we are constructing a special off-line ESI source where free radicals can be sprayed together with peptides and proteins. Following collection of the spray, we plan to analyze the "spot" by either a second ESI experiment, coupled with MS/MS, or by MALDI and either PSD or MS/MS. Preliminary data show that the approach is feasible and that we are able to generate and react OH radicals with various peptides and small proteins. We intend to continue this developm ent as part of a general strategy to use contemporary ionization methods in MS as means for determining chemical and biochemical reactivity and as means of following interactions and conformation change. We are also considering a device incorporating vortex flows to help conduct the reactions and also to better focus the ions produced by electrospray.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们有兴趣开发质谱方法来跟踪和揭示生物材料的化学性质。我们希望在“现场”进行这项工作，即在进行多边土地退化指数或电喷雾电离层的过程中进行。自由基损伤是一个领域，其中相当多的推论证据指出自由基在损伤DNA和蛋白质中的后果。我们相信，通过在电喷雾源中进行破坏性的化学反应，可以获得生物材料与自由基反应的直接证据。为此，我们正在构建一个特殊的离线ESI源，其中自由基可以与肽和蛋白质一起喷射。喷雾收集后，我们计划分析的“斑点”，无论是第二个ESI实验，再加上MS/MS，或通过MALDI和PSD或MS/MS。初步数据表明，该方法是可行的，我们能够产生和反应OH自由基与各种肽和小蛋白质。我们打算继续这一发展的一部分，使用现代电离方法在MS作为手段，用于确定化学和生物化学反应性，并作为以下的相互作用和构象变化的手段。我们还在考虑一种结合涡流的装置，以帮助进行反应，并更好地集中电喷雾产生的离子。