Scale-up feasibility of plasma deposition in 3D tissue engineering scaffolds

3D 组织工程支架中等离子体沉积放大的可行性

• 批准号: EP/E010962/1
• 负责人: James Bradley
• 金额: $ 16.02万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE010962%2F1
• 关键词: Scale; up; feasibility; plasma; deposition

The field of Tissue Engineering offers the possibility of generating replacement organs and tissue in response to loss or failure with obvious application in human health care.One of the many challenges that must be met to achieve wide application of this principle is the provision of guidance to cell positioning in the early stages of tissue formation. A common strategy is to support cells using synthetic scaffolds, designed to degrade gradually leaving cells supported by the extra cellular matrix that they produce naturally over time. The problem of initial poor cell adhesion to synthetic polymers commonly used in tissue engineering and poor ingress of cells into the scaffold centre has been widely reported in the literature. One method (proof-of-principle) that has been explored at Nottingham is to promote cell adhesion and ingress by chemically functionalising the scaffold using plasma deposition. However, to date the characteristic penetration depth (e-folding length) of the deposit from the scaffold periphery is only about 1 mm. In this feasibility study, we shall attempt to develop a low pressure pulsed polymerising plasma struck in monomers such as allyl amine which has the characteristics (plasma parameters in on and off time of the pulse) necessary to allow penetration of the plasma into pours of reduced dimensions and over much large distances. This will allow a scale up from small scale scaffold discs treated currently to real-world scaffolds for cartilage, bone or other large tissue engineering applications.

组织工程领域提供了产生替代器官和组织的可能性，以响应在人类健康护理中的明显应用的损失或失败。要实现这一原理的广泛应用，必须满足的许多挑战之一是在组织形成的早期阶段提供细胞定位的指导。一种常见的策略是使用合成支架来支持细胞，这些支架被设计成逐渐降解，使细胞由它们随着时间的推移自然产生的细胞外基质支持。组织工程中常用的合成聚合物的初始不良细胞粘附和细胞进入支架中心的不良问题已在文献中广泛报道。在诺丁汉已经探索的一种方法（原理验证）是通过使用等离子体沉积化学官能化支架来促进细胞粘附和进入。然而，迄今为止，存款从支架周边的长度（e-折叠长度）仅为约1 mm。在该可行性研究中，我们将尝试开发一种在单体如烯丙基胺中撞击的低压脉冲聚合等离子体，（在脉冲的开和关时间中的等离子体参数），这是允许等离子体穿透到减小尺寸的浇注物中并且穿透大得多的距离所必需的。这将允许从目前处理的小规模支架盘到用于软骨、骨或其他大型组织工程应用的真实世界支架的按比例放大。

项目成果

A role of ions in thin film deposition in a pulsed acrylic acid RF discharge

脉冲丙烯酸射频放电中离子在薄膜沉积中的作用

• 发表时间: 2007
• 作者: N/a Voronin

Investigation of cell-surface interactions using chemical gradients formed from the diffusion of depositing species in polymerizing plasma

使用聚合等离子体中沉积物质扩散形成的化学梯度研究细胞表面相互作用

• 发表时间: 2007
• 作者: N/a Zelzer

Plasma Polymer Gradients and Their Use for Cellular Guidance

等离子体聚合物梯度及其在细胞引导中的应用

• 发表时间: 2006
• 作者: N/a Zelzer

3T3 Fibroblast Cell-Surface Interactions Using Chemical Gradients Formed by Plasma Polymerization

3T3 使用等离子体聚合形成的化学梯度的成纤维细胞表面相互作用

• 发表时间: 2008
• 作者: N/a Bradley