SYNTHETIC & ANALYTICAL

合成的

• 批准号: 7305795
• 负责人: CHARLES R IDEN
• 金额: $ 28.37万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7305795
• 关键词: 2' -deoxyadenosine; Aristolochic Acids; Arts; Biological; Cells; Chemicals; Core Facility; DNA; DNA biosynthesis; DNA chemical synthesis; Detection; Development; Fluorescence; High Pressure Liquid Chromatography; Human Resources; Improve Access; Individual; Inorganic Sulfates; Investigation; Kidney; Kidney Diseases; Maps; Mass Spectrum Analysis; Methods; Mus; National Center for Research Resources; Peptides; Progress Reports; Proteins; Proteomics; Rate; Rattus; Relative (related person); Reporting; Research; Research Personnel; Resources; Rodent Model; Sampling; Schedule; Score; Services; Site; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Standards of Weights and Measures; Study Section; System; Techniques; Technology; Testing; Time; United States National Institutes of Health; Unspecified or Sulfate Ion Sulfates; Work; adduct; analog; analytical method; aristolochic acid I; aristolochic acid II; chemical synthesis; detector; experience; instrument; instrumentation; mass spectrometer; metabolic abnormality assessment; novel; phosphoramidite; programs

a pure sample as a standard. New peptide adducts formed by AL-II have been isolated by HPLC using a fluorescence detector and analyzed by MALDI mass spectrometry. A brief report of each of these advances is found in the Progress Report/Preliminary Studies section. Finally, Dr. Iden, seeking to improve access to state-of-the-art mass spectrometry techniques, submitted a proposal for a new triple quadrupole mass spectrometer system to the NIH National Center for Research Resources. Review by the Center for Scientific Review Special Emphasis Panel in July, 2006 was highly favorable and a priority score of 170 was assigned to the proposal. Council is scheduled to meet in October, 2006. If this instrument is acquired in the Spring, 2007, as scheduled, levels of sensitivity for quantitative detection of DNA and protein adducts will be increased by a factor of ten over that attained by our current triple quadrupole instrumentation. A. Specific Aims Oligodeoxynucleotide synthesis and chemical analysis performed by the Core Facility provides fundamental support for all Program research. The Core will provide modified and normal oligomers to all investigators. DNA synthesis personnel will work closely with synthetic chemists to test new, modified deoxynucleosides phosphoramidites and incorporate them into DNA oligomers. We will refine our quantitative mass spectrometer methods and proteomics capabilities and focus on the development of sensitive, new techniques to identify DNA and protein adducts formed by the aristolochic acids (AAs) and their metabolites. The specific aims of the Core Facility are: 1. Toprepare

一 纯样品作为标准品。AL-II形成的新肽加合物已通过HPLC分离， 荧光检测器检测并通过MALDI质谱法分析。以下是对这些进展的简要报告。 请参阅进度报告/初步研究部分。最后，伊登博士，寻求改善获得 国家的最先进的质谱技术，提交了一份新的三重四极质谱的建议， 光谱仪系统的NIH国家研究资源中心。科学中心的审查 2006年7月的评审特别强调小组非常有利，优先得分为170分， 这个提议理事会定于2006年10月举行会议。如果在2007年春季获得该仪器， 按照计划，定量检测DNA和蛋白质加合物的灵敏度将提高 是我们目前的三重四极杆仪器所能达到的十倍。 A.具体目标 由核心设施进行的寡脱氧核苷酸合成和化学分析提供了基本的 支持所有项目研究。核心将向所有研究者提供修饰和正常低聚物。 DNA合成人员将与合成化学家密切合作，测试新的、经过修饰的脱氧核苷 亚磷酰胺并将其掺入DNA寡聚体中。我们将改进我们的定量质谱仪 方法和蛋白质组学能力，并专注于开发敏感的新技术， 马兜铃酸及其代谢产物形成的DNA和蛋白质加合物。 核心基金的具体目标是： 1.托普雷什