CORE--CHEMISTRY

描述：(申请人描述)核心部分的主要目标是(1)建立化学分析和寡核苷酸合成的集中设施；(2)为受邀演讲者组织一系列研讨会，介绍与计划成员兴趣有关的研究；以及(3)为计划参与者提供行政支持。首席调查员协调计划的行政方面，召集咨询委员会，安排系列研讨会，并促进参与者之间的沟通。核心项目的科学功能由伊登博士指导，包括复杂的高效液相和质谱仪分析以及自动化DNA合成和纯化。质谱仪将在分析含有新的DNA加合物和合成中间体的寡聚脱氧核苷酸方面发挥关键作用。两个重要的新仪器，一个是微质量四元系LC/MS/MS系统，一个是微质量平台LC/MS仪器，最近加入了质谱学设施，将是这些分析的基础。寡聚脱氧核苷酸的合成和提纯对于该计划中的所有研究人员都是至关重要的；这是作为一个集中设施最有效地完成的。在核心设施中包括研究的分析和综合方面，确保所有项目研究都可以免费获得。

DESCRIPTION: (APPLICANT'S DESCRIPTION) The primary objectives of the core component are (1) to establish centralized facilities for chemical analysis and oligodeoxynucleotide synthesis; (2) to organize a seminar series for invited speakers to present research related to the interests of Program members; and (3) to provide administrative support for Program participants. The Principal Investigator coordinates administrative aspects of the Program, convenes the Advisory Committee, arranges the seminar series, and facilitates communication between the participants. The scientific functions of the Core Project are directed by Dr. Iden and include sophisticated HPLC and mass spectrometer analysis and automated DNA synthesis and purification. Mass spectrometry will play a critical role in the analysis of oligodeoxynucleotides containing new DNA adducts and synthetic intermediates. Two important new instruments, a Micromass Quattro LC/MS/MS system and a Micromass Platform LC/MS instrument, were recently added to the Mass Spectrometry Facility and will be fundamental for these analyses. Synthesis and purification of oligodeoxynucleotides is of essential importance for all investigators in the Program; this is most effectively accomplished as a centralized facility. Including the analytical and synthetic aspects of the research in the core facility ensures that it is freely available for all project research.