CORE--CHEMISTRY

核心--化学

• 批准号: 6563825
• 负责人: CHARLES R IDEN
• 金额: $ 11.52万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563825
• 关键词: adduct; analytical chemistry; biomedical facility; high performance liquid chromatography; information dissemination; mass spectrometry; nuclear magnetic resonance spectroscopy; nucleic acid chemical synthesis; synthetic nucleic acid; synthetic nucleotide

DESCRIPTION: (APPLICANT'S DESCRIPTION) The primary objectives of the core component are (1) to establish centralized facilities for chemical analysis and oligodeoxynucleotide synthesis; (2) to organize a seminar series for invited speakers to present research related to the interests of Program members; and (3) to provide administrative support for Program participants. The Principal Investigator coordinates administrative aspects of the Program, convenes the Advisory Committee, arranges the seminar series, and facilitates communication between the participants. The scientific functions of the Core Project are directed by Dr. Iden and include sophisticated HPLC and mass spectrometer analysis and automated DNA synthesis and purification. Mass spectrometry will play a critical role in the analysis of oligodeoxynucleotides containing new DNA adducts and synthetic intermediates. Two important new instruments, a Micromass Quattro LC/MS/MS system and a Micromass Platform LC/MS instrument, were recently added to the Mass Spectrometry Facility and will be fundamental for these analyses. Synthesis and purification of oligodeoxynucleotides is of essential importance for all investigators in the Program; this is most effectively accomplished as a centralized facility. Including the analytical and synthetic aspects of the research in the core facility ensures that it is freely available for all project research.

产品说明：核心部分的主要目标是（1）建立化学分析和寡脱氧核苷酸合成的集中设施;（2）组织一系列研讨会，邀请演讲者介绍与计划成员利益相关的研究;（3）为计划参与者提供行政支持。首席研究员协调该计划的行政方面，召集咨询委员会，安排系列研讨会，并促进参与者之间的沟通。 核心项目的科学功能由Iden博士指导，包括复杂的HPLC和质谱仪分析以及自动化DNA合成和纯化。 质谱法将在分析含有新的DNA加合物和合成中间体的寡脱氧核苷酸中发挥关键作用。两个重要的新仪器，一个Micromass Quattro LC/MS/MS系统和一个Micromass Platform LC/MS仪器，最近被添加到质谱设施，将是这些分析的基础。 寡脱氧核苷酸的合成和纯化对该计划的所有研究人员都至关重要;这是最有效的集中设施。 将研究的分析和综合方面包括在核心设施中，确保所有项目研究都可以免费使用。