SYNTHETIC & ANALYTICAL

合成的

• 批准号: 8069938
• 负责人: CHARLES R IDEN
• 金额: $ 32.23万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-05 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8069938
• 关键词: 2' -deoxyadenosine; Aristolochic Acids; Arts; Biological; Cells; Chemicals; Core Facility; DNA; DNA biosynthesis; Detection; Development; Fluorescence; High Pressure Liquid Chromatography; Human Resources; Improve Access; Individual; Inorganic Sulfates; Investigation; Kidney; Kidney Diseases; Maps; Mass Spectrum Analysis; Methods; Mus; National Center for Research Resources; Peptides; Progress Reports; Proteins; Proteomics; Rattus; Relative (related person); Reporting; Research; Research Personnel; Resources; Rodent Model; Sampling; Schedule; Services; Site; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Study Section; System; Techniques; Technology; Testing; Time; United States National Institutes of Health; Unspecified or Sulfate Ion Sulfates; Work; adduct; analog; analytical method; aristolochic acid I; aristolochic acid II; chemical synthesis; detector; experience; instrument; instrumentation; mass spectrometer; meetings; metabolic abnormality assessment; novel; phosphoramidite; programs

a pure sample as a standard. New peptide adducts formed by AL-II have been isolated by HPLC using a fluorescence detector and analyzed by MALDI mass spectrometry. A brief report of each of these advances is found in the Progress Report/Preliminary Studies section. Finally, Dr. Iden, seeking to improve access to state-of-the-art mass spectrometry techniques, submitted a proposal for a new triple quadrupole mass spectrometer system to the NIH National Center for Research Resources. Council is scheduled to meet in October, 2006. If this instrument is acquired in the Spring, 2007, as scheduled, levels of sensitivity for quantitative detection of DNA and protein adducts will be increased by a factor of ten over that attained by our current triple quadrupole instrumentation. A. Specific Aims Oligodeoxynucleotide synthesis and chemical analysis performed by the Core Facility provides fundamental support for all Program research. The Core will provide modified and normal oligomers to all investigators. DNA synthesis personnel will work closely with synthetic chemists to test new, modified deoxynucleosides phosphoramidites and incorporate them into DNA oligomers. We will refine our quantitative mass spectrometer methods and proteomics capabilities and focus on the development of sensitive, new techniques to identify DNA and protein adducts formed by the aristolochic acids (AAs) and their metabolites. The specific aims of the Core Facility are: 1. Toprepare

一个 以纯样品为标准。用高效液相色谱分离了AL-II形成的新的多肽加合物 荧光检测器，用MALDI质谱仪进行分析。每一项进展的简要报告如下 可在进度报告/初步研究部分找到。最后，伊登博士，寻求改善对 最先进的质谱学技术，提交了一份关于新的三重四极杆质量的提案 美国国立卫生研究院国家研究资源中心的光谱仪系统。理事会定于2006年10月举行会议。如果这台仪器是在2007年春季收购的， 按计划，DNA和蛋白质加合物定量检测的灵敏度水平将提高a 比我们目前的三重四极子仪器高出十倍。 A.具体目标 核心设施进行的寡核苷酸合成和化学分析提供了基本的 为所有计划研究提供支持。Core将向所有研究人员提供改良的和正常的低聚物。 DNA合成人员将与合成化学家密切合作，测试新的、经修饰的脱氧核苷 亚磷酰胺，并将其加入DNA低聚物中。我们将改进我们的定量质谱仪 方法和蛋白质组学能力，并专注于开发敏感的、识别 由马兜铃酸(AAs)及其代谢产物形成的DNA和蛋白质加合物。 核心基金的具体目标是： 1.最重要的准备