Acquisition of an Applied Biosystems 4000 QTRAP Mass Spectrometer

描述（由申请人提供）：本提案为购买Applied Biosystems 4000 QTRAP质谱仪和数据系统申请资金。该仪器直接连接到提供LC/MS/MS能力的Eksigent纳米高效液相色谱系统，并将位于石溪医学院的蛋白质组学中心。需求现在已经超过了在中心进行质谱/质谱实验的能力。该系统将解决这一关键问题，并将显著促进石溪大学（SBU）的8位杰出科学家的研究目标和实验室生产力，他们构成了主要用户群。其他生物医学研究人员也可以作为工具使用。这种质谱仪系统将使这些研究人员直接获得分析生物分子的重要新技术，这些技术目前在SBU无法获得，从其他组织也无法获得或难以获得。LC/MS/MS对于具有重要生物医学意义的分子的表征是无与伦比的，包括脂质，生物聚合物，如肽，寡核苷酸，寡糖和较小的非挥发性，不稳定或热不稳定的化合物。通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术，现在可以方便、快速地对这些物质进行常规分析。新的依赖信息的数据采集程序允许在单次注射中对多种化合物进行全面表征。采用敏感的LC/MS/MS技术的定量方法对一些研究人员来说是必不可少的。该仪器将由蛋白质组学中心训练有素的人员操作和维护，主任将安排日常操作并为用户提供技术专业知识。一个咨询委员会将确保与主要用户和其他小灵通支助的调查人员取得联系，并将负责该仪器的长期运行。创新性生物医学研究需要经常使用最新的分析技术，以提高对人类疾病的认识，并为这些疾病提供新的治疗方法。应用生物系统4000 QTRAP仪器将允许对许多生物分子进行低水平分析，这对美国国立卫生研究院支持的研究人员在Stony Brook的研究工作至关重要。他们将使用该仪器提供的新的分析技术来探索导致糖尿病、癌症和心脏病的机制，并帮助为每种疾病状态定义新的生化标志物和药物。

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an Applied Biosystems 4000 QTRAP Mass Spectrometer and Data System. The instrument is linked directly to an Eksigent nano HPLC system affording LC/MS/MS capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. Demand has now exceeded the ability to perform MS/MS experiments in the Center. This system will address this critical problem and will significantly promote the research objectives and laboratory productivity of eight distinguished scientists from Stony Brook University (SBU) who comprise the major users group. Additional biomedical research investigators will also have access as instrument. This mass spectrometer system will make directly available to these investigators important new techniques for the analysis of biomolecules, which are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non- volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. New information dependent data acquisition routines permit full characterization of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument. Relevance Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Applied Biosystems 4000 QTRAP instrument will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for each disease state.