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Acquisition of an Applied Biosystems 4000 QTRAP Mass Spectrometer

购买 Applied Biosystems 4000 QTRAP 质谱仪

基本信息

批准号：
7214942
负责人：
CHARLES R IDEN
金额：
$ 43.46万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2008-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7214942
关键词：
Acquisition Applied Biosystems 4000 QTRAP

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an Applied Biosystems 4000 QTRAP Mass Spectrometer and Data System. The instrument is linked directly to an Eksigent nano HPLC system affording LC/MS/MS capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. Demand has now exceeded the ability to perform MS/MS experiments in the Center. This system will address this critical problem and will significantly promote the research objectives and laboratory productivity of eight distinguished scientists from Stony Brook University (SBU) who comprise the major users group. Additional biomedical research investigators will also have access as instrument. This mass spectrometer system will make directly available to these investigators important new techniques for the analysis of biomolecules, which are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non- volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. New information dependent data acquisition routines permit full characterization of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument. Relevance Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Applied Biosystems 4000 QTRAP instrument will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for each disease state.

描述（由申请人提供）：本提案要求为购买应用生物系统4000 QTRAP质谱仪和数据系统提供资金。该仪器直接连接到提供LC/MS/MS能力的Eksigent纳米HPLC系统，并将位于斯托尼布鲁克医学院的蛋白质组学中心。现在的需求已经超过了在中心进行MS/MS实验的能力。该系统将解决这一关键问题，并将显着促进研究目标和实验室生产力的八位杰出的科学家从斯托尼布鲁克大学（SBU）谁构成的主要用户组。其他生物医学研究调查员也将作为工具访问。该质谱仪系统将使这些研究人员直接获得用于分析生物分子的重要新技术，这些技术目前在SBU不可用，并且无法从其他组织获得或难以获得。LC/MS/MS在表征具有重要生物医学意义的分子方面是无与伦比的，包括脂质、生物聚合物（如肽、寡核苷酸和寡糖）以及非挥发性、不稳定或热不稳定的较小化合物。通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术现在允许对这些物质进行简单快速的常规分析。新的信息依赖的数据采集程序允许在一个单一的注射过程中的多种化合物的全面表征。采用灵敏的LC/MS/MS技术的定量方法对一些研究人员来说是必不可少的。该仪器将由蛋白质组学中心训练有素的人员操作和维护，主任将安排日常操作并为用户提供技术专业知识。一个咨询委员会将确保接触主要用户和其他PHS支持的调查人员，并将负责仪器的长期运行。创新的生物医学研究需要经常获得最新的分析技术，以提高对人类疾病的认识，并为这些疾病提供新的治疗方法。应用生物系统4000 QTRAP仪器将允许对许多生物分子进行低水平分析，这些生物分子对斯托尼布鲁克的NIH支持的研究人员的研究工作至关重要。他们将使用该仪器提供的新的分析技术来探索导致糖尿病，癌症和心脏病的机制，并帮助定义每种疾病状态的新生化标志物和药物。