Synthetic and Analytical Chemistry

合成和分析核心项目通过为所有研究人员提供修饰的寡聚脱氧核苷酸和分析服务，构成了该计划中所有研究项目的基础。该核心的目标是(A)合成代表内源性DNA损伤的修饰脱氧核苷，并将它们转化为5‘-O-DMT-3’-O-亚磷酰胺，以并入寡聚脱氧核苷酸；(B) 使用自动DNA合成技术生成高纯度、定点修饰的寡核苷酸；以及(C)为该计划的所有参与者提供基本的分析服务，包括质谱学、高效液相色谱和毛细管电泳法。此外，核心设施将开发方案研究和最先进设施运行所需的新的合成方法和分析技术。在一个由训练有素的人员运营的核心设施中，将计划研究的综合和分析方面结合起来，将确保所有计划参与者及时获得改良的寡脱氧核苷酸，并确保主要设备项目可用并正常运行。

The Synthetic and Analytical Core Project forms the underpinning for all research projects in the Program by furnishing modified oligodeoxynucleotides and analytical services for all investigators. The objectives of the Core are (a) to synthesize modified deoxynucleosides that represent endogenous DNA damage and to convert them to the 5'-O-DMT-3'-O-phosphoramidites for incorporation into oligodeoxynucleotides; (b) to generate highly pure, site-specifically modified oligodeoxynucleotides using automated DNA synthesis techniques; and (c) to provide essential analytical services, including mass spectrometry, HPLC, and capillary electrophoresis, for all participants in the program. In addition, the Core Facility will develop new synthetic methods and analytical techniques needed for Program research and for the operation of a state-of-the-art facility. Combining the synthetic and analytical aspects of Program research in a Core facility operated with highly trained personnel will guarantee timely access to modified oligodeoxynucleotides for all Program participants and ensure that major equipment items are available and functioning.