ACQUISITION OF A MALDI/TOF MASS SPECTROMETER SYSTEM

购买 MALDI/TOF 质谱仪系统

• 批准号: 6292201
• 负责人: CHARLES R IDEN
• 金额: $ 32.43万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292201
• 关键词: biomedical equipment purchase; matrix assisted laser desorption ionization

This proposal requests funds for the purchase of a PE Biosystems Voyager-DE STR MALDI/TOF Mass Spectrometer and Data System. This high resolution instrument is equipped with a reflectron analyzer and is capable of accurate mass analysis of molecular ions and fragment ions forms through post source decay. It will be located in the SUNY- Stony Brook Mass Spectrometer Facility and will significantly promote the research objectives and laboratory productivity of fourteen distinguished scientists from SUNY-Stony Brook and Brookhaven National Laboratory who comprise the major users group. The mass spectrometer system will make directly available to these investigators important new techniques for the analysis of biomolecules which are presently unavailable locally and inaccessible or difficult to obtain from other organizations. MALDI/TOF is unsurpassed for the structural analysis of molecules with critical biomedical consequences, including biopolymers such as proteins, oligonucleotides, and oligosaccharides and a diverse group of compounds that are non-volatile, labile, or thermally unstable. Accurate mass analysis of protein molecular ions and determination of proteolytic fragments from proteins will be employed to identify proteins by automated computer searching internet-based databanks. Negative ion detection for oligodeoxynucleotides, modified oligomers, and DNA-protein complexes will be essential for several investigators. Automated sample analysis from large multi-well plates will ensure high throughput and optimal utilization of the instrument. The instrument will be operated and maintained by trained personnel at the USB Mass Spectrometer Facility, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument. The Stony Brook administration has made a significant contribution toward the purchase of the instrument and to the installation and operation over its expected lifetime.

该提案要求提供资金，用于购买一台PE生物系统Voyager-DE STR MALDI/TOF质谱仪和数据系统。这台高分辨率仪器配备了反射分析器，能够准确地对分子离子和通过源后衰变形成的碎片离子进行质量分析。它将设在纽约州立大学石溪分校的质谱计设施中，并将极大地促进来自纽约大学石溪分校和布鲁克海文国家实验室的14名杰出科学家的研究目标和实验室生产率，他们是主要用户群体。质谱计系统将使这些研究人员能够直接获得用于生物分子分析的重要新技术，这些技术目前在当地无法获得，也无法从其他组织获得或难以从其他组织获得。MALDI/TOF在具有关键生物医学后果的分子的结构分析方面是无与伦比的，包括生物聚合物，如蛋白质、寡核苷酸和寡糖，以及一系列非挥发性、不稳定或热不稳定的化合物。将采用蛋白质分子离子的准确质量分析和蛋白质中蛋白质降解片段的测定，通过计算机自动搜索互联网数据库来鉴定蛋白质。对一些研究人员来说，负离子检测寡聚脱氧核苷酸、修饰低聚物和DNA-蛋白质复合体将是必不可少的。来自大型多孔板的自动化样品分析将确保仪器的高通量和最佳利用率。该仪器将由受过培训的USB质谱计设施人员操作和维护，主任将安排日常操作并为用户提供技术专长。一个咨询委员会将确保接触主要用户和其他由小灵通支助的调查人员，并负责该仪器的长期运作。石溪管理部门为购买该仪器以及在其预期寿命内安装和运行做出了重大贡献。