CORE--IMAGING

这一建议的假设集中在PKD遗传模型中的分子和细胞水平的缺陷。肾小管上皮细胞被认为是肾囊肿发生发展的主要病理实体。虽然在组织之外对细胞的研究存在局限性，但细胞测量提供了许多复杂的技术，以高空间和时间分辨率测量活细胞中的分子功能，从而可以提供最终可以在体内模型中验证的分子证据。为了建立疾病的细胞水平模型，我们需要各种各样的测量方式来将特定的遗传缺陷与特定的细胞表型联系起来。这项提案中使用的许多技术可以为特定细胞途径中的缺陷提供关键的分子证据。成像核心设施提供了一套独特的、互补的基于显微镜的 能够对活细胞中的分子功能进行高分辨率测量的设备。

The hypotheses of this proposal are focused on the molecular and cellular level deficits in genetic models of PKD. The epithelia of the renal tubule are thought to be the primary pathological entity in the development of renal cysts. While there are limitations to the study of the cell outside of its tissue context, cellular measurements provide access to a number of sophisticated techniques to measure molecular function in living cells at high spatial and temporal resolution and can thus provide molecular evidence that ultimately can be verified within in vivo models. To build cellular level models of disease we require a wide variety of measurement modalities to relate specific genetic defects to a specific cellular phenotype. Many of the techniques used in this proposal can provide key molecular evidence for deficits in specific cellular pathways. The Imaging Core facility provides a unique and complementary set of microscopy-based modalities that can make high-resolution measurements of molecular function in living cells.