STRUCTURE FUNCTION RELATIONSHIPS IN POLYSACCHARIDES

多糖的结构功能关系

• 批准号: 7726003
• 负责人: SRINIVAS JANASWAMY
• 金额: $ 0.39万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726003
• 关键词: Behavior; Biopolymers; Complex; Computer Retrieval of Information on Scientific Projects Database; Depth; Fiber; Food; Funding; Glycosaminoglycans; Goals; Grant; Institution; Lateral; Least-Squares Analysis; Link; Methods; Molecular; Molecular Structure; Numbers; Pattern; Pharmacologic Substance; Plants; Polymers; Polysaccharides; Process; Research; Research Personnel; Resources; Solutions; Solvents; Source; Specimen; Stretching; Structure; Structure-Activity Relationship; System; Temperature; United States National Institutes of Health; Wood material; analog; aqueous; galactoglucomannan; gellan; glucuronoxylomannan; lambda Carrageenan; molecular modeling; programs; solute; three dimensional structure; xanthan gum

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our long term goals are to elucidate the molecular structures of (non-crystalline) biopolymers, especially polysaccharides, and their interactions with solvent and solute molecules and the synergistic interactions in mixed polysaccharides towards an in depth understanding of their functional behavior. In this regard, the main thrust of this proposal is centered on determining the three-dimensional structures of a number of biologically important and industrially useful polysaccharides. Our ongoing study will include, but not limited to (as need arises), the specimens from algal (iota, kappa and lambda carrageenans); bacterial (gellan analog and cepacian); fungal (glucuronoxylomannan); plant (rhamnogalacturonan); wood (galactoglucomannan) and a binary system (bacterial xanthan:plant glucomannan and bacterial acetan:plant glucomannan). These molecules or molecular complexes have an inherent tendency to form helical structures with only limited lateral ordering and thus cannot be grown as single crystals. Hence, fiber diffraction is the only method of choice for visualizing their three-dimensional organization. Such structural information is needed for understanding their interactions with solvent and solute molecules and with other polysaccharides for their effective utilization in food and pharmaceutical applications. The results will further highlight the conformational space available to polysaccharides as they occur in glycosaminoglycans and cellular walls. Oriented and polycrystalline fibers will be obtained from aqueous polymer solutions through stretching process at room temperature. Linked-Atom Least-Squares (LALS) program will be used to build the molecular models to be in conformity with the diffraction pattern and to solve the structure.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的长期目标是阐明（非结晶）生物聚合物，特别是多糖的分子结构，及其与溶剂和溶质分子的相互作用以及混合多糖中的协同相互作用，以深入了解其功能行为。在这方面，该提案的主旨集中在确定许多具有生物学意义和工业用途的多糖的三维结构。我们正在进行的研究将包括但不限于（根据需要）藻类标本（iota、kappa 和 lambda 角叉菜胶）；细菌（结冷胶类似物和洋葱）；真菌（葡萄糖醛酸木甘露聚糖）；植物（鼠李半乳糖醛酸）；木材（半乳葡甘露聚糖）和二元系统（细菌黄原胶：植物葡甘聚糖和细菌乙酰：植物葡甘聚糖）。 这些分子或分子复合物具有形成仅具有有限横向排序的螺旋结构的固有倾向，因此不能生长为单晶。因此，纤维衍射是可视化其三维组织的唯一选择方法。需要此类结构信息来了解它们与溶剂和溶质分子以及与其他多糖的相互作用，以便在食品和制药应用中有效利用它们。结果将进一步强调多糖在糖胺聚糖和细胞壁中存在时可用的构象空间。 将聚合物水溶液通过室温拉伸工艺得到取向多晶纤维。链接原子最小二乘（LALS）程序将用于建立符合衍射图样的分子模型并求解结构。