DVMT OF METHODS, INSTR & TECH FOR IMPROVED PROTEOME & PROTEIN COMPLEXES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aim 2 seeks to improve proteome analyses through the development of new methods, instrumentation, and techniques. Specific areas of focus included improved methods for dealing with the phosphoproteome; advances in MS sensitivity; elution time prediction for improved protein identification; and advances in top-down intact protein measurements. These capabilities are the key to any study that involves discovery proteomics for clinical samples. Progress in this area has been driven by collaborative projects that feature non-conventional and/or highly complex samples and the need for dynamic range (e.g., Rossie, Klemke, Squier, D. Smith, Katze, Warren, and Kulkarni).