DVMT OF METHODS, INSTR & TECH FOR IMPROVED PROTEOME & PROTEIN COMPLEXES

方法的 DVMT，导师

• 批准号: 7721387
• 负责人: RICHARD D SMITH
• 金额: $ 69.63万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-08 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721387
• 关键词: Area; Clinical; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Grant; Institution; Measurement; Methods; Proteins; Proteome; Proteomics; Range; Research; Research Personnel; Resources; Sampling; Source; Techniques; Time; United States National Institutes of Health; improved; instrumentation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aim 2 seeks to improve proteome analyses through the development of new methods, instrumentation, and techniques. Specific areas of focus included improved methods for dealing with the phosphoproteome; advances in MS sensitivity; elution time prediction for improved protein identification; and advances in top-down intact protein measurements. These capabilities are the key to any study that involves discovery proteomics for clinical samples. Progress in this area has been driven by collaborative projects that feature non-conventional and/or highly complex samples and the need for dynamic range (e.g., Rossie, Klemke, Squier, D. Smith, Katze, Warren, and Kulkarni).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 具体目标 2 旨在通过开发新方法、仪器和技术来改进蛋白质组分析。 具体重点领域包括处理磷酸化蛋白质组的改进方法； MS 灵敏度的进步；预测洗脱时间以改进蛋白质鉴定；以及自上而下完整蛋白质测量的进展。 这些功能是任何涉及临床样本发现蛋白质组学研究的关键。 该领域的进展是由具有非常规和/或高度复杂样本以及动态范围需求的合作项目推动的（例如，Rossie、Klemke、Squier、D. Smith、Katze、Warren 和 Kulkarni）。