COMPOSITION ANALYSIS OF 6-114 AND 6-284 BY HPAEC

HPAEC 对 6-114 和 6-284 进行成分分析

• 批准号: 7722663
• 负责人: Parastoo Azadi
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722663
• 关键词: Anions; Calibration; Chromatography; Computer Retrieval of Information on Scientific Projects Database; Condition; Equation; Funding; Grant; Hydrolysis; Injection of therapeutic agent; Institution; Melanocytic nevus; Mole the mammal; Monosaccharides; Numbers; Research; Research Personnel; Resources; Sampling; Solutions; Source; Standards of Weights and Measures; Time; Trifluoroacetic Acid; United States National Institutes of Health; Vial device; Water; prevent; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Composition analysis by HPAEC The samples were hydrolyzed to release the monosaccharides and were analyzed by High pH-Anion-Exchange Chromatography (HPAEC). Hydrolysis of dried samples was performed with 400 ¿L of 2 N trifluoroacetic acid (TFA) at 100¿C for 4 h. Also, a 400-¿L blank TFA was hydrolyzed under the same conditions. After hydrolysis, the samples were lyophilized and resuspended in 150 ¿L of nanopure water, sonicated in cold water for 7 min and transferred to an injection vial. For analysis, the autosampler was set to deliver 10 ¿L of sample or standard solution per injection. Blanks (water) were injected between standards and the sample to prevent carry-over contamination. A mix of sugar standards with a known number of moles was hydrolyzed at the same time as the samples. Three concentrations of standards (0.5, 1.0, and 2.0 nmoles per injection) were prepared and injected to establish a calibration equation. The number of moles of each sugar was quantified by linear interpolation from the calibration equation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 HPAEC法成分分析 样品水解释放单糖，并通过高pH阴离子交换法进行分析 色谱法（HPAEC）。 干燥样品的水解用400 μ L的2 N三氟乙酸（TFA）进行， 100 ℃下4小时。 此外，在相同条件下水解400 L空白TFA。 水解后，将样品 冻干并重悬于150 μ L纳米纯水中，在冷水中超声处理7分钟，并转移至注射液中 小瓶。 对于分析，将自动进样器设置为每次进样输送10？L样品或标准品溶液。 空白（水） 在标准品和样品之间注射，以防止残留污染。 一种混合糖标准， 已知摩尔数的水溶液与样品同时水解。 三种浓度的标准品（0.5，1.0， 和每次注射2.0纳摩尔）并注射以建立校准方程。 每种物质的摩尔数 通过校准方程的线性内插来定量糖。