CSHL Cell and Developmental Biology of Xenopus

CSHL 细胞和非洲爪蟾的发育生物学

基本信息

批准号：
7631032
负责人：
TERRI I. GRODZICKER
金额：
$ 5.99万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7631032
关键词：
Actins Administrator Animal Model Antibodies Area Arts Binding Biochemistry Bioinformatics Biology Books Cell membrane Cells Communities Complement Complex Coupled Cytoskeleton DNA Data Development Developmental Biology Developmental Cell Biology Developmental Process Drosophila pros protein Eating Educational Curriculum Educational process of instructing Embryo Ensure Equipment Evaluation Exercise Exposure to Faculty Feedback Funding Future Gene Transfer Techniques Genes Genetic Genetic Screening Genomics Growth Factor Growth Factor Interaction Health Home environment Hour Image Imagery In Situ Hybridization Individual Injection of therapeutic agent Institution Instruction Knowledge Laboratories Laboratory Assistant Lead Learning Left Manuals Maps Mesoderm Methodology Methods Microdissection Microscope Molecular Molecular Analysis Morphogenesis Morphology Oocytes Operative Surgical Procedures Phase Postdoctoral Fellow Preparation Procedures Process Protocols documentation Publishing Rana Reagent Reporter Research Research Personnel Research Project Grants Research Technics Resolution Resources Route Running Schedule Seminal Series Shapes Source Student Selections Students Suggestion System Techniques Technology Testing Time Tissue Grafts Tissues Training Transgenic Organisms United States National Institutes of Health Vendor Videotape Work Xenopus Xenopus laevis cDNA Library cell motility data mining design digital egg embryo tissue expectation experience gene discovery graduate student improved instructor interest investigator training lecturer lectures lens meetings moesin notochord novel phosphorothioate programs public health relevance research study response skills success time use xenopus development

项目摘要

DESCRIPTION (provided by applicant): Dramatic progress has been made in the fields of cell and developmental biology in recent years, largely due to the application of new technical approaches to classical problems. This has led to the need for advanced courses to train new investigators to study those animal models, such as the frog, Xenopus, that have been important in making new discoveries. Funds are being sought to continue the Cold Spring Harbor Laboratory course on "Cell and Developmental Biology of Xenopus", which has been taught for fifteen years as part of an integrated set of courses in a number of areas of modern biology. This course provides extensive laboratory exposure to the biology and manipulation of embryos from the frogs Xenopus laevis and Xenopus tropicalis. This course prepares students for independent research in the cell, genetic, and molecular analysis of vertebrate development. It introduces the unique attributes of Xenopus, which allow the functional analysis of developmentally important genes, whilst at the same time introducing the major technologies of modern molecular and cellular developmental biology, in a system that allows direct manipulation of embryos and visualization of developmental processes, such that at the end of the course, students are ready for cutting edge research. The Cell and Developmental Biology of Xenopus course is designed to train students how to take advantage of some unique qualities. It is often difficult for investigators to initiate studies of Xenopus development on their own because the techniques for raising embryos or manipulating them are unfamiliar or because their background knowledge of developmental biology is inadequate. This course is designed to provide students with a concentrated and repeated exposure to the most important research techniques, and the researchers doing them, so that they can initiate their own independent research projects after returning to their home institutions. The course provides an intensive exposure, over a ten-day period, to the biology and manipulation of Xenopus embryos. The course also provides a resource network that the students can easily access after they have returned to their home laboratories. Through these routes, the course trains investigators at the advanced graduate, postdoctoral, and faculty levels to use Xenopus in their research. PUBLIC HEALTH RELEVANCE: It is anticipated that the central organization of the course and some of the basic course material will remain unchanged during the proposed period of support. However, new and fast-developing topics will be discussed and integrated into the course as the field progresses.

描述（由申请人提供）：近年来在细胞和发育生物学领域取得了巨大进展，这在很大程度上是由于在经典问题上应用了新的技术方法。这导致需要高级课程来培训新的研究人员来研究这些动物模型，例如青蛙，Xenopus，这些模型对于做出新发现很重要。正在寻求资金继续有关“ Xenopus的细胞和发育生物学”的冷春港实验室课程，该课程已在许多现代生物学领域的一组综合课程中教授了15年。本课程可广泛接触到青蛙Xenopus laevis和xenopus tropicalis的生物学和操纵。本课程使学生在脊椎动物发育的细胞，遗传和分子分析中进行独立研究。它介绍了爪蟾的独特属性，该属性允许对发育重要的基因进行功能分析，同时介绍现代分子和细胞发育生物学的主要技术，在直接操纵胚胎和可视化发育过程的系统中，学生可以在课程的最终研究中进行剪切研究。 Xenopus课程的细胞和发育生物学旨在培训学生如何利用某些独特的品质。研究人员通常很难自行启动Xenopus发育的研究，因为提高胚胎或操纵它们的技术是不熟悉的，或者因为他们对发育生物学的背景知识不足。本课程旨在为学生提供对最重要的研究技巧的集中和重复接触，研究人员这样做，以便他们在返回家庭机构后可以启动自己的独立研究项目。该课程在为期十天的时间内对Xenopus胚胎的生物学和操纵提供了密集的暴露。该课程还提供了一个资源网络，学生返回家庭实验室后可以轻松访问。通过这些路线，该课程培训了高级毕业生，博士后和教职员工级别的调查人员，以在他们的研究中使用Xenopus。公共卫生相关性：预计该课程的中央组织和一些基本课程材料将在拟议的支持期间保持不变。但是，随着领域的进展，将讨论新的和快速发展的主题。