WEST NILE VACCINE FOR NON-HUMAN PRIMATES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目标是测试一种候选水泡性口炎疫苗，用于保护恒河猴免受西尼罗河病毒(WNV)感染。在TNPRC，36%的室外非人类灵长类(NHP)群体对西尼罗河病毒抗体阳性。商店样本的比较表明，这些动物是在2002年感染的。这些数据表明，疫苗将有助于保护NHP群体免受感染。 六只印度恒河猴被分配到这个项目中。两只动物在2006年接种了西尼罗河病毒IV疫苗，并于2007年再次挑战。在接种前和接种后采集血样，用RT-PCR和抗体检测监测疾病进展。脑脊液从两只动物身上采集，淋巴活检从一只动物身上采集。监测小鼠对西尼罗河病毒感染的免疫应答。 在该项目的疫苗接种挑战环节，四只动物于2007年5月接种了疫苗，并在30天后进行了免疫接种。30天后，所有四只动物都接受了挑战。虽然检测到了抗体，但WNV在恒河猴中的低抵抗力排除了对疫苗效力的评估。VSV载体在小鼠中的应用结果已发表。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to test a candidate vesicular stomatitis vaccine for protection of rhesus macaques against West Nile Virus (WNV) infrection. At the TNPRC 36% of the outdoor non-human primate (NHP) colony were found to be antibody positive for WNV. Comparisons of store samples indicated that the animals had been infected in 2002. These data indicate that a vaccine would be helpful in protecting NHP colonies from infection. Six Indian rhesus macaques were assigned to this project. Two animals were inoculated IV with WNV in 2006 and re-challenged in 2007. Blood samples were collected prior to and post inoculation to monitor disease progression using RT-PCR and antibody assays. CSF was collected from both animals, lymph node biopsies were collected from one animal. The immune response to WNV infection was monitored. For the vaccination challenge arm of the project, four animals were vaccinated in May of 2007 and boosted 30 days later. All four animals were challenged on 30 days later. Although antibody was detected, the low pathegenecity of WNV in rhesus precluded an evaluation of vaccine efficacy. Results with VSV-vector in mice were published.